Gastric Cancer Of Differentially Expressed Genes To Identify And Ems1 Protein Expression Of The Clinical And Pathological Significance

Part 1 Study on the Gene Expression Profile of poorly differentiated Gastric Carcinoma Using cDNA MicroarrayObjective:The study was to establish the gene expression profile of poorly differentiated gastric carcinoma, isolate gastric carcinoma-related genes, and primary investigate the relationship between gastric carcinoma-related genes and gastric carcinoma.Methods:The changes of gene expression profile between poorly differentiated gastric carcinoma and adjacent normal tissue of gastric epithelia were analyzed by cDNA microarray which representing approximately 10000 genes in this study. Based on expression analysis of this cDNA microarray , subsequently, genes that were differentially expressed in poorly differentiated gastric carcinoma and adjacent normal tissue of gastric epithelia were identified by bioinfornation.Results: 212 genes that were differentially expressed in cancer and non- cancerous tissues were identified; 169 genes were highly expressed >2.0-fold in cancer tissues, 43 genes were lower expressed >2.0-fold in cancer tissues. In cancer tissues, genes related to cell cycle, growth factor and cell motility were highly expressed. In non-cancerous tissues, genes related to gastrointestinal-specific function and immune response was highly expressed.Conclusion: These results not only established the gene expression profile of poorly differentiated gastric carcinoma but also a useful clue for future cloning gastric cancer related genes. Part 2 Clinicopathological significance of Expression of EMS1 Gene in gastric carcinomaObjective: To study the relationship between expression of EMS1 gene and carcinpogenesis, invasion and metastasia.Methods: EMS1 protein expression in 146 cases of gastric carcinoma and 20 cases of normal gastric mucosa was examined by S-P immunohistochemical method. Futhermore, the relationship between the expression of EMS1 and the clinicopathologic features was determined.Result :EMS1 protein displayed in cytoplasmic localization. The positive rate of EMS1 protein expression was 20% (4/20) in normal gastric mucosa. The positive rate of EMS1 protein expression was 68.42% (26/38) in intraepithelial neoplasia,but in the gastric carcinoma the positive rate of EMS1 protein expression was 89.72% (131/146). The positive rate of EMS1 protein expression of gastric carcinoma was significantly higher than that in intraepithelial neoplasia (P<0.001) . The positive rate of EMS1 protein expression in intraepithelial neoplasia was significantly higher than that in normal gastric mucosa (P<0.001). The positive rate of EMS1 protein expression was 60.87%(14/23) in early stage was significantly lower than that in 95.12%(117/123)advance stage. The EMS1 positvely in 96.81%(91/94)of 94 pairs of samples from primary carcinoma and their lymph node metastases was significantly higher than that in 76.97% (40/52)carcinoma without lymph node metastases.There was no association between EMS1 protein expression and sex, age, differentiation and diameter.Conclusions: It is indicated that EMS1 protein expression is related to carcinogenesis, lymph node metastasis and clinical stage in gastric carcinoma. Part 3 Clinicopathological significance of Expression of EMS1 Gene in hepatocelluar carcinomaObjective: To study the relationship between expression of EMS1 gene and carcinpogenesis, invasion and metastasia.Methods: EMS1 protein expression in 78 cases of HCC and 8 cases of normal liver tissue was examined by S-P immunohistochemical method. Futhermore, the relationship between the expression of EMS1 and the clinicopathologic features was determined.Result :EMS1 protein displayed in cytoplasmic localization. The positive rate of EMS1 protein expression was 89.69% in HCC. The positive rate of EMS1 protein expression was 30.26% in their corresponding paracancerous tissues and nomal tissues(p<0.05). The positive rate of EMS1 protein expression of HCC with liver cirrhosis was significantly higher than that in HCC without liver cirrhosis (P<0.05).The positive rate of EMS1 protein expression in HCC with venous tumor emboli was significantly higher than that without venous tumor emboli (P<0.05). There was significant difference in Edmondson's histopathological classification (P<0.05). There was no association between EMS1 protein expression and sex, age and diameter.Conclusions: It is indicated that EMS1 protein expression is related to carcinogenesis,liver cirrhosis and venous tumor emboli and histopathological classification in hepatocelluar carcinoma.