| OBJECTIVE: By detecting the different expression levels of the LIMK1 and uPAR in colorectal cancer, normal tissue adjacent to cancer and colorectal adenomas, analyze the different expressions of them in the benign and malignant colorectal tissues. Find out the relationship between them in colorectal cancer and with the clinical data such as age,pathological classification, the Dukes stage, lymph node metastasis, to clear the mechanism of lymphatic metastasis,and whether they can be used as the prognostic marker of colorectal cancer, and whether they can provide a new therapeutic targets for controling tumor lymphatic metastasis.Methods: The 87 cases of the colorectal cancer, 26 cases of colorectal adenoma and 34 cases of normal tissue were collected. All diagnose and classification were performed according to the WHO(2003) colorectal tumor histologic classification. By manual tissue chip, with a needle we biopsy a standard histologic section , placed the core into an array on a recipient paraffin block, fuse, slice, gaine and bake.Immunohistochemistry method was used to examine the expression of LIMK1and uPAR in colorectal cancer, adenoma and normal tissue. Spss16.0 was applied to analyze the data, significance was set at P<0.05.Results: 3 tissue chips (5×10) were made. The significant tissue structures were watched in the other dots. The rate of complete tissue dots was 97.21%. The HE staining was uniformity and no dropping, moving and wrinkling. The immunohistochemical results showed that the positive site was accurate and the background was clear. The positive rates of LIMK1 in normal tissue, adenoma and colorectal cancer were 20.58%, 38.46% and 75.86%, respectively, and the difference among groups was significant (P<0.05). Normal tissue showed no positive staining of uPAR(0.00%), and there was significantly differentially expressed uPAR in adenoma (11.53%) compared with that in colorectal cancer (37.93%) (P<0.05). There was no significantly correlation between age and the expression of LIMK1 and uPAR (P>0.05) but regarding to tumor size, the expression of LIMK1 and uPAR in the <5.0cm group was significantly lower than in the≥5.0cm group (57.14% and 20.00%, 88.46% and 50.00%)(P<0.01). These cases of colorectal cancer were divided into high, middle and low differentiation groups by pathological classification. The expression positive rates of LIMK1 and uPAR increased as differentiation grade decreasing(40.00% and 20.00%,70.02% and 31.91%,96.67% and 63.33%)and the significant difference between groups existed (P<0.01). Expression positive of uPAR and LIMK1 in lymph node-positive group was significantly higher than that in the negative group (97.78% and 55.56%, 52.39% and 19.48%) (P<0.05). Between groups of colorectal cancer of C, D stage and A, B stage by Dukes stage criterion, there were significantly different positive rates of LIMK1 and uPAR(89.13% and 52.17%,60.97% and 21.95%). In the colorectal cancer, the positive rate of the uPAR in the LIMK1 positive group was higher than in LIMK1 negative group(35.63%/2.30%) and correlation analysis showed there was direct correlation between LIMK1 and uPAR in colorectal cancer tissues.(r>0, P<0.01).Conclusions:1. The expression of LIMK1 and uPAR is closely related to pathological classification, lymph node metastasis and clinical stage in colorectal cancer.2. LIMK1 and uPAR are closely related to lymph node metastasis and positively correlated with each other in colorectal cancer. |
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