| Gold nanoparticles had many special properties such as quantum size effects, surface effects, optical effects and compatibility effects. With these properties, gold nanoparticles were widely used in the fields of clinical medicine, materials and more. Moreover, in the field of medical tests, now gold nanoparticles labels had been the forth important markers. Gold nanoparticles show the potential applied value because of their special physical and chemical characteristics. At present, the analytical applications of gold nanoparticles are as optical probes or as electrochemical sensors after chemical modification. However, there are rarely reports that spectrophotometry or Resonance Rayleigh scattering method is used to the determination of pharmaceuticals with unmodified gold nanoparticle probes. Main content are listed as follow:The preparation and characterization methods, spectroscopy characterization and analytical application of gold nanoparticles and the property, purpose and the present situation of its analysis of 6-mercaptopurine are revivwed here.A novel spectrophotometry method has been developed for the determination of micro amounts of 6-mercaptopurine, employing gold nanoparticles as probes. The color of test solution changed to blue from bright red is significantly observed upon the gold nanoparticles self-assembled with 6-mercaptopurine and the absorption peak was obviously different. Before reaction, there is a plasmon band at 520 nm that is the characteristics of gold nanoparticles and 6-mercaptopurine has no absorption peak in rang of visible light region. After reaction, the peak of 520 nm is lower, in addition, there is a maximum absorption peak located at 680 nm, which is the characteristic peak of the binding product. The absorption at 680 nm of different concentrations of 6-mercaptopurine could be quantified with good linear, with a low detection limit of 17μg/L, under optimal conditions. The relative standard deviation (n=11) was 2.9%at 0.25 mg/L 6-mercaptopurine concentration level. The proposed method was successfully applied to determination of the drug spiked human urine. The results are satisfactory, which indicates that the method is not only sensitive, simply, but also reliable and suitable for practical application.A novel Resonance Rayleigh scattering method was developed for the determination of micro amounts of 6-mecaptopurine, employing gold nanoparticles as probes. In pH 5.50 weak acid medium, the greatly enhanced Resonance Rayleigh scattering spectrum can be observed upon the gold nanoparticles self-assembled with 6-Mercaptopurine because both of them react with each other to form a binding product with bigger volume. In this work, The Resonance Rayleigh scattering spectral characteristics, optimum conditions for the reaction and the influencing factors were investigated. The relative Resonance Rayleigh scattering intensity (ΔIRRS) is directly proportional to the concentration of 6-Mercaptopurine in the range of 0.017-0.32 mg/L. The method has high sensitivity and the limit of detection for 6-Mercaptopurine is 8μg/L. The RSD (n=11) is 5.1% at 0.12 mg/L 6-mercaptopurine concentration level. The effect of coexisting substances on the determination of 6-mercaptopurine was studied and the results showed that this method had good selectivity. Therefore, the proposed method is successfully used to determine 6-mercaptopurine in human urine by Resonance Rayleigh scattering with gold nanoparticle probes. |
PDF Full Text Request