Measurement Of Inter-alpha Inhibitor Protein, Interleukin-8 And C-reactive Protein In Neonatal Bacterial Infections And Its Clinical Significance

Objective To study the change of inter-alpha inhibitor protein (I a IP), interleukin-8 (IL-8) and c-reactive protein (CRP) in neonatal bacterial infections,to analyze the test value of these three markers in early diagnosis of neonatal bacterial infections,to explore clinical significance of the markers when united with each other.Method A total of 64 newborns who were treated in our pediatrics outpatient and wards of newborn as well as obstetrical department were enrolled in the study.44 neonates whose course of disease was smaller than 12 hours were divided into bacterial infection group (n=22) and non-infection group (n=22). Serum IαIP,IL-8 and CRP were measured when they were hospitalized and at 24h from the beginning of diseases as well as recovery period. Another 20 healthy newborns were served as control group and were detected only once. IL-8 was measured by enzyme-linked immunosorbent assay while CRP was detected by immune rate nephelometry.We used western blot to detect IαIP.Results The level of serum IL-8 in infection group rose obviously within 12h and its sensitivity reached 81.82%, but it went down notably at 24h. While its specificity reached 95.45% both within 12h and 24h. The level of serum CRP in infection group rose slightly within 12h and had the lowest sensitivity, but it reached 77.27% at 24h. While its specificity was 90.91% and 81.82% within 12h and 24h respectively. The sensitivity of serum I a IP was the highest both within 12h and 24h when compared with IL-8 and CRP singly, while its specificity both exceeded 80%.When three markers were united with each other, IL-8 in combination with IαIP had the highest sensitivity, while its specificity was 77.27% and 81.82% within 12h and 24h respectively. IαIP in combination with CRP had sensitivity of 95.45% within 12h and 86.36% at 24h, but had the lower specificity. IL-8 in combination with CRP had the specificity of 83.86% and 81.82% within 12h and 24h respectively, but its sensitivity was lower than the other two united markers.Conclusion1.When measured singly, IL-8 has the highest specificity of 95.45% both within 12h and 24h. IαIP has the highest sensitivity of 95.45%within 12h and 81.82% at 24h.CRP has the medium sensitivity.and specificity.2.When three markers were united with each other,IL-8 in combination with I a IP has the highest sensitivity of 95.45% both within 12h and 24h, while its specificity is 77.27% and 81.82% within 12h and 24h respectively. So using IαIP and IL-8 as markers of early diagnosis in neonatal bacterial infections is reliable.