Experimental Research Of The Effect Of Apigenin On The Invasion And Mentastasis Of Human Colon Cancer Cell Sw480 In Vitro

Objective: To investigate the effect of apigenin on the invasion and metastasis abilities of human colon cancer cell SW480 in vitro;Methods: Human colon carcinoma cell line SW480 were incubated in vitro, using cell biology techniques. The morphological changes of SW480 cell were investigated through light microscope and electron microscope; the effect of apigenin on SW480 cell was studied by means of MTT; Flowcytometry was used to study the pro-apoptosis effects of apigenin on SW480 cells; and the invasion of cells was tested using Transwell method;Results: The amount of human colon carcinoma cells treated by apigenin decreased, compared with that of controls. Among normal cells, apoptotic corpuscles could be detected by electron microscope. Apigenin inhibited the growth of human colon carcinoma cell line SW480 in a time-dependent and dose-dependent manner. Analysis of Flowcytometry results revealed that with the increase of apigenin using in SW480 cells, the apoptosis rate of human colon carcinoma cells was improved; Transwell method showed that Apigenin significantly inhibit the invasion and migration of SW480 cells in does-dependent manner.Conclusions: In vitro, apigenin can efficiently inhibit the proliferation, invasion and migration of SW480 cells, in a time-dependent and dose-dependent manner, and induce the apoptosis of SW480 cells. So Apigenin is a potential drug to anti-invasion and anti-migration of tumor cells.