Investigation And Analysis Of Taiyuan, Shanxi Region Of P. Aeruginosa And Metallo Enzyme Gene Research

Objective:(1) To investigate the distribution and antimicrobial resistance of pseudomonas aeruginosa in nosocomial, to supply referrence for clinical use of drugs rationally. (2) In the study, to discuss occurrence of metallo-β-lactamase (MBL) in clinical isolates of pseudomonas aeruginosa in the area, for MBL strains, to supply a effective supervision in the clinical laboratory, and the spread and dissemination of MBL will be prevented.Methods:Clinical isolates of pseudomonas aeruginosa were retrospectively analyzed and antimicrobial susceptibility were tested by K-B method. The data were analyzed by WHONET 5.4 software. Collected 64 strains of imipenem-resistant pseudomonas aeruginosa from hospital. The test strains were screened by EDTA-IPM disk diffusion and EDTA double disk synergy method, to confirm the MBLs gene by PCR method.Results:A total of 432 clinical isolates of Pseudomonas aeruginosa were collected,88.4% were from the hospitalized patients and were mainly distributed in departments of respiration, neurology and intensive care unit (ICU),73.9% from sputum tract; The Pseudomonas aeruginosa isolates showed the lowest resistance rate (10.0%) to amikacin, the resistance rates to antimicrobial agents from low to high were ceftazidime (17.2%), ciprofloxacin (31.2%), meropenem (32.3%), cefepime (32.5%), imipenem (35.3%), cefoperazone-sulbactam (38.1%), the resistance rates to other antimicrobial agents were higher than 40%.64 strains of IRPA were mainly distributed in respiration and neurosurgery, most from sputum tract.14 strains were detected through EDTA method. Among them,3 and 2 strains showed specific IMP-1 and VIM-2 gene respectively by PCR.Conclusions:Pseudomonas aeruginosa is the major pathogenic bacteria in nosocomial infection, the multidrug resistance is very serious and its resistance mechanisms are very complicated. (1) Pseudomonas aeruginosa were recovered from patients in the respiratory, the isolates were mainly recovered from sputum tract. (2) Pseudomonas aeruginosa to antimicrobial resistance rate remained at a relatively high level. (3) Metallo-β-lactamase (MBL) was the vital imipenem-resistant mechanism of Pseudomonas aeruginosa, other mechanisms may exist.