| Hepatic fibrosis is a kind of disease that when liver cells stimulation and inflammation of dead, the extracellular matrix lose its balance,so lead to abnormal growths fibrous connective tissue inside liver pathological process. Liver fibrosis is not an independent disease, it's a kind of chronic liver disease and the important pathologic features of the liver disease. Also is the important development to cirrhosis intermediate links. In recent years, as medical molecular biology technology application, the pathogenesis of hepatic fibrosis carried on the thorough study, and achieved a lot of important achievements,such as:1) Normal and fibrosis liver extracellular matrix components characteristics; 2) liver fibrosis process is an important source of extracellular matrix; 3) clarify the key of liver fibrosis cell source of cytokines, adjust mode and the intracellular signaling pathways; 4) extracellular matrix synthesis gene regulation; 5) key substrate protease and inhibitory factor characteristic; 6) apoptosis adjustment role of astrocytes quantity of determination and hepatic stellate cell apoptosis of liver fibrosis role reversal to reveal.Objectives:To review and summarize the current understanding and treatment of traditional Chinese and Western medicine on hepatic fibrosis, to observe Huo Xue pill's influence on the immune hepatic fibrosis rat's body weight, liver and spleen weight, liver function (ALT, AST, TBA), TGF-β1,α-SMAand pathological changes of liver tissues induced by porcine serum, to observe anti-fibrosis effect of Huo Xue pill (HXP)and the mechanism.Methods:100 Vistar female rats were randomly divided into five groups (n=20). In model group, porcine serum 0.5 ml was injected into abdominal cavity, twice per week, incessantly for ten weeks. The rest four groups, except for normal control group which was through intraperitoneal injection of normal saline, were given the same as model group and gastric perfusion with Huo Xue pill (thirty-five fold dose of adult recommended dosage), and colchicines tablets (thirty-five fold dose of adult recommended dosage), respectively for once per day. For model and normal control groups, they were administered with distilled water 2ml by gavage. Adjustment of the amount of dose was based on weight of rats each week.At the end of 10 weeks, stop modeling.10 rats from each group were sacrificed. In order to observe conditions of body weight, weight of liver and spleen, ascites, liver function, TGF-β1,α-SMAand pathological changes of liver tissue, a series of preparations should be done, like Weighing rats, fixing in supine position after anesthetization of 10% hydration chlorine aldehyde, taking out blood from carotid artery, opening abdominal cavity to observe ascites and to weigh liver and spleen, obtaining 1.5cm*1cm*0.5cm size or so liver tissue in the same position in the right lobe of liver fixed by formalin, embedded sections by paraffin, and stained by H. E.The rest rats from each group stopped modeling, and continued the hxp treatment for ten weeks. At the end of 20 weeks, sacrifice all of the rats, and the concrete operation was the same as the end of 10 weeks'. Not only observing conditions of body weight, weight of liver and spleen, ascites, liver function, TGF-β1,α-SMA, and pathological changes of liver tissue, but also comparing indexes with the end of ten weeks'. Criteria for grading and staging of chronic hepatitis were based on the revised pathological diagnostic criteria of chronic hepatitis (seen in attached table 1) in China, which was on the foundation of Scheuer programs. The liver tissue stained with H. E., its pathological changes were observed under 60 times light microscope.Results:When the experiment was over, there was no obvious ascites in each group. There were no significant differences in body weight, weight of liver and spleen of each group in both 10 weeks and 20 weeks (P>0.05). At the end of 10 weeks, model group AST, ALT, TBA, TGF-β1,α-SMA were significantly higher than normal group (P<0.01), invigorate the intervention group and normal hxp group without difference (P>0.05), and significantly lower than colchicine tablet group (P <0.01); At the end of 20 weeks, the model group, hxp group 10 weeks AST, ALT, TGF-β1,α-SMAwere significantly higher than normal group (P<0.01), invigorate the piece medication 10 weeks group and colchicines tablets group (P>0.05 no difference), invigorate the piece medication 20 weeks group AST, TGF-β1,α-SMA below autumn narcissus alkali group (P<0.05). Experimental 20 weeks and experimental 10 weeks, invigorate the piece of drug 10 weeks between the AST, ALT, TGF-β1,α-SMA no difference (P>0.05), invigorate the drug group 20 weeks group tablet between ALT, alpha SMA reduce no difference (P>0.05), the AST, TGF-beta 1 tvb-n, reduce have difference (P<0.05). Experimental 10 weeks, model rats lobular architecture disorder, collect abbacy expanded significantly, fiber interval wider, collagen density, partly visible lobular structure disorder and false flocculus to form; Huoxue pill intervention group and colchicines tablets group of liver tissue visible fibre hyperplasia, but degree is model group light, fiber interval; less, fine Experimental 20 weeks, and the model groupliver tissue pathological changes when than 10 weeks without opparent change medication 20 weeks, invigorate the slice of fibre hyperplasia degree is 10 weeks, some were significantly reduce the basic normal pathology. Diagnostic criteria according to chronic hepatitis (see the appendixa), invigorate the piece intervention group were significantly lighter liver fibrosis degree in model group, three groups of fibrosis scores are significant difference (10 weeks P=0.00 20 weeks P= 0.02). And invigorate the slice 20 weeks group and 10 weeks group fibrosis scores in experimental 20 weeks compared with experimental 10 weeks significantly (P <0.01). Huoxue pill 20 weeks group, colchicines tablets experiments 20 weeks and experimental 10 weeks between fibrosis scores group have difference (P< 0.05).Conclusion:Huoxue pill can restrain the rats induced swine serum immunity fibrosis, its curative effect and treatment time have correlation. Huoxue pill of liver fibrosis can inhibit the rat liver TGF-β1,α-SMA expression, this function is the mechanism of huoxue pill. |
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