Electro-acupuncture Treatment After Sciatic Nerve Injury In The Spinal Cord Of Bfgf And Bdnf, Cntf And Ngf Expression,

【Purpose】By Electroacupuncture treatment of sciatic injure rats, using muscles-sciatic nerve-spinal cord pathways to observe the variation of the nerve growth factor. Discussion the mechanisms of Electroacupuncture treatment in nerve injure.【Method】First,88 rats were randomly divided into normal group 24, model group 24, model control group 24, Electroacupuncture treatment group 16. Modeling method for modeling the sciatic nerve clamping, the first skin incision after anesthesia, muscle blunt dissection, exposing the sciatic nerve, full deduction clamping time 5s, the damage caused by long-2mm point, and then sterilized suture layer by layer. Electroacupuncture treatment the huantiao,Yinmen,Yanglingquan,Chengshan 4 point to the damage side. By Electroacupuncture treatment apparatus, current 2mA, frequency 5Hz, Sparse-dense waves.Derived three times.The first seven days after the damage repair on the modeling. Derived for the second ten days after acupuncture treatment.Derived to the third twenty times after acupuncture treatment.Derived to take part nerves, muscles, spinal cord, three parts of the qualitative semiquantitative immunohistochemical observation Muscle wet weight measurement, neuronal staining, to understand how electroacupuncture for its tissue repairing situation, the derived behavior prior to testing, to understand the functional recovery of the situation.【Result】1 Behavioral observation1.1 Incline plane testAfter Electroacupuncture treatment 10 times Electroacupuncture treatment group is better than the model group and model control group, and the Electroacupuncture treatment group differences compared with the model group, the results have statistical significance. The Result is the same in 20 times that the angle of the Electroacupuncture treatment group is better than model group and model control group. After Electroacupuncture treatment showed increased muscle strength.1.2 Heat pain threshold testIn Electroacupuncture treatment after 10 times Electroacupuncture treatment group less than the model group, the difference was not significant, and Electroacupuncture treatment after 20 times that Electroacupuncture treatment group is better than model control group, but the difference is not obvious. After Electroacupuncture treatment showed improvement in trend.2 The repair of muscle2.1 Gastrocnemius wet weightAfter Electroacupuncture treatment 10 times, Electroacupuncture treatment group gastrocnemius wet weight is better than the model group and model control group, and after Electroacupuncture treatment 20 times is also Electroacupuncture treatment group better than model control group and model group, but no significant difference, indicating that Electroacupuncture can prevent the effects of muscle atrophy.2.2 Muscle cell diameterAfter the electrical acupuncture 10 times the diameter of gastrocnemius of Electroacupuncture treatment group is better than model group and model control group, and after 20 times Electroacupuncture treatment that Electroacupuncture treatment group still better than the model group and model control group. The Electroacupuncture treatment group has statistically significant with model group. That Electroacupuncture could make the gastrocnemius muscle tissue repair. 3 Immunohistochemical comparisons between groups Through immunohistochemical determination of the manner of the nerve growth factors in content of the spinal cord 3.1 THE optical density analysis of bFGF immunohistochemistryAfter 10 times by Electroacupuncture treatment, Electroacupuncture treatment group shows the optical density significantly in model group and control group, Electroacupuncture treatment group compared to the control group is statistically significant. Electroacupuncture treatment group after Electroacupuncture 20 treatment times the optical density analysis is better than model control group and model control group, in which Electroacupuncture treatment group and model group include statistical significance. Electroacupuncture has enhanced the role of bFGF expression.3.2 THE optical density analysis of NGF immunohistochemistryAfter 10 times by Electroacupuncture treatment, Electroacupuncture treatment group shows the optical density significantly which model group and model control group, and the electroacupuncture treatment group compared model group and control group was statistically significant. After Electroacupuncture treatment 20 times Electroacupuncture treatment group is better than model group and control group, but not statistically significant. Electroacupuncture has enhanced the role of NGF expression. 3.3 THE optical density analysis of BDNF immunohistochemistryAfter 10 times by Electroacupuncture treatment, Electroacupuncture treatment group shows the optical density significantly is better than model group and model control group and normal group, and Electroacupuncture group of model group and model control group is statistically significant. However, in the Electroacupuncture treatment of 20 times the optical density analysis revealed that from large to small is the model group> Electroacupuncture group> normal group> control group, no rules exist. Electroacupuncture for the role of BDNF in the treatment period was 10 times higher expression.3.4 THE optical density analysis of CNTF immunohistochemistryAfter 10 times by electroacupuncture treatment, Electroacupuncture treatment group shows the optical density significantly is better than model group and model control group and normal group, and statistically significant. However, Electroacupuncture treatment after 20 times the level of Electroacupuncture group expressed is lowest in each group, the expression of CNTF that Electrical for the most affecting time in Electroacupuncture treatment of 10 times.【Conclusion】1. Electroacupuncture can make inclined plane test rats muscle strength enhancement.2. Electroacupuncture on Heat pain threshold test rats has improvement trend.3. Electroacupuncture on the role of muscle cells diameter to promote recovery, also visible in the gastrocnemius muscle wet after Electroacupuncture treatment has increased.4. Electroacupuncture on nerve growth factor promoted the expression, according to the Immunohistochemical experiments show that Electroacupuncture can enhance nerve recovery.