| Objective:Wujiwan composed of three herbs such as Coptis,Evodia rutaecarpa(Juss.)Benth,Paeonia lactiflora Pall. The major functions of Wujiwan are liver-fire attacking stomach, stomach-ache, acid requrqitation, bitter taste and diarrhea. Ultra-fine powder technology for compound preparation can enchance the effective component content thing preparation and bioavailability, this article were determined by HPLC method in already shot the content of berberine hydrochloride, conveniently, accurately reflect the shot of the effective components already to rat doing animal pharmacokinetic, organization distribution and discharge experiment, respectively for Wujiwan and superfine pill irrigation, determine its stomach blood medicine density, calculate dynamic parameters of statistical analysis and comparison and study for the further research and the body already ultrastructural Wujiwan bioavailability and clinical application provides experimental basis.Methods:For subjects in rats with conventional e respectively, has shot and superfine Wujiwan irrigation stomach, determined by HPLC method index component berberine hydrochloride content, determine its blood drug concentration, already shot in calculating rats in vivo pharmacoki-netic, organization distribution and discharge parameters, statistical ana-lysis and comparison for further research, already pill body ultra bioa-vailability and clinical application provides experimental basis.1, pharmacokinetic experiment:with conventional e yourself pills and sup-erfine e rats irrigation has shot stomach, by reveed-phase HPLC index component berberine hydrochloride, the blood drug concentration cal-culated pharmacokinetic software related parameters, a comparative study. 2, the organization distribution experiment:choose berberine hydrochlo-ride as index, berberine by reveed-phase HPLC in rats in vivo in rats and berberine, the concentration of each organization changes over time. Rat stomach conventional irrigation has e has shot blasting and superfine e after the concentration discretion in organizations. Then compare conve-ntional e f pill group and superfine e of the experimental data has bolus group, utrasmall e f pill group compared with conventional e in the org-anization has bolus group dissolubility concentration and curve area under the AUC whether a certain degree of increased.3 Excretion experiment:rats after filling in certain stomach, collect rats time period of bile, muck, urine. Determination of blasting in them has e excretion rates, and a cumulative excretion rate. The above experiments contrast research for further research on the body already Ultra-fine powder pill for bioa- vailability and clinical application has positive significance.Results:1 Through to ordinary e f pills and superfine e dynamic learning experimental comparison has the pills were pleasant, etc doses of superfine e after filling in stomach has shot at a point in time were greater than the conventional blood medicine density has pills, Ultra-fine powder Cmax, has shot groups are more routine group AUC value increases, utrasmall group compared with conventional group of Cmax and AUC value increased by 15.7% respectively, and the index and 12.8% a peak time and other ingredients are not shown chemical changes significantly. Specifications dose of the drug, utrasmall Chinese medicine's effective blood drug concentration than conventional preparation medicine blood drug concentration, related to the high concentration of chemical composition is increased.2 In radicles draining experiments are both excretion rates were lower, explain e in bile irrigation has shot in the stomach and the two experimental excrete less without obvious difference. 3 In dung and urine excreta experimental study, found that e is primarily through dung has shot, while urinary excretion way in little. Drain The above experiments contrast research for further research on the body already pill ultrastructural e bioavailability and clinical application has positive significance. Conclusion:By using ultra-fine powder technology in the preparation of wujiwan, Superfine grinding technology used in the preparation of cyclopentanone himself after blasting, improving the effective components has shot dissloution degrees. Through comparing conventional pentosan content determination of blasting and superfine e has the group has shot blasting group, utrasmall e in blood has feces, mainly drug concentration is higher than conventional wujiwan. |
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