Content And Its Impact Factor Of Pod Fern Flavonoids

Matteuccia struthiopteris belongs to Onocleaceae.It is a widespread temperate ferns, grows in the valley of wetland forests, stream side of the wetlands, between 80-3000m above sea level are the growth of a variety of value can be medicinal, edible and as ornamental plants. Matteuccia struthiopteris have complex chemical composition, in which flavonoids are a very important category. In this paper, Matteuccia struthiopteris foliage leaves as research subjects of different regions and different periods of growth Matteuccia struthiopteris foliage leaves content of flavonoids in studies, and Matteuccia struthiopteris foliage leaves of soil contained in the determination of mineral elements, combined with The growth of environmental conditions, and finally to organize and comprehensive evaluation of flavonoids content differences and environmental factors, for further research is a plant chemical classification and Matteuccia struthiopteris habitat protection, rational exploitation and to provide evidence and reference. The main conclusions of experiments are as follows:1. Matteuccia struthiopteris foliage leaves in the extraction of flavonoids and optimization of detection methods, and ultimately determine the optimal extraction method for accurately weighed Matteuccia struthiopteris powder 1.000g, add 30ml 90% ethanol, at 80℃；water bath pot reflux extraction lh, returning after removing hydrochloric acid solution by adding 2ml lh. Remove to cool to room temperature, filtration, the filtrate vacuum evaporated with methanol to volume lOmL volumetric flask, shake, use the filter membrane. Provide optimal conditions for high performance liquid chromatography column:Shimadzu C18 (250×4.6 mm,5μm); mobile phase was methanol (A)-0.2% phosphoric acid (B); using linear gradient elution (0â†'30min, methanol 40%â†'65%,0.2% phosphoric acid 60%â†'35%); flow rate 1.0mL/min; column temperature is 30℃; detection wavelength 360nm; injection volume 20μL.2. Through April to September Matteuccia struthiopteris foliage leaves collected in the determination of total flavonoids obtained Matteuccia struthiopteris content of total flavonoids content in different collection periods with very significant difference, the maximum difference was 2.9 times. Different regions collected Matteuccia struthiopteris Determination of Flavonoids in the results show that different regions collected Matteuccia struthiopteris foliage leaves Flavonoids there were significant differences taken from the Qinling Mountains in Shaanxi 4 to the differences were less taken from Gansu, Henan, taken from the highest levels of Changbai Mountains in Jilin. 3. The Matteuccia struthiopteris foliage levels and sensitive growth in the determination of soil mineral elements in different regions, show in its acquisition of the Matteuccia struthiopteris leaves and the soil nutrients contained in the mineral elements measuring element bigger, what difference except the P, Mn outside, other elements were higher than the average contents of these elements in plants average content. Acquisition of the different regions in Matteuccia struthiopteris foliage leaves containing mineral elements of its growth in the soil mineral elements contained in some elements have a certain correlation. Fe, Cu content with Mg a very significant positive correlation, the content of Cu and Mg is a significant positive correlation. Matteuccia struthiopteris of enrichment mineral elements, the differences of enrichment coefficients is P Fe 50 times above. Analyzing different gathering area of fern pods mineral elements is likely due to the difference of contents in pods environmental conditions of different sensitive fern grows in the growth process, in order to adapt to the environment better growth, the demand for the different elements and its genetic factors sensitive Matteuccia struthiopteris to decide their absorptive capacity of each mineral elements of different.4. Sunshine duration and latitude and flavonoids content in Matteuccia struthiopteris foliage leaves respectively, positive correlation with annual average temperature sensitive flavonoids content in Matteuccia struthiopteris foliage leaves respectively have negative correlation, lower average annual temperature longer sunshine time is to promote pods fern vegetative leaves the flavonoids content accumulation of important factors. Mineral elements in Matteuccia struthiopteris foliage leaves Fe, Cu content and flavonoids content in Matteuccia struthiopteris foliage leaves were very significant negative correlation. In the natural growth environment, each factors on the role of Matteuccia struthiopteris comprehensive function, the environment is mutual constraint, mutual factors influence, very complicated.