Separation And Purification Of Angelica Sinensis And Houttuynia Injection Of Qualitative And Quantitative Analysis

With the high-speed development of science and technology and progress of human society, the advancement of modernization of traditional Chinese medicines provides a broad space for the development of traditional Chinese medicine industry. The thesis just takes traditional Chinese medicines as our research object. In this work, the effective components of a commonly used traditional Chinese medicine, called Radix Angelicae, were separated and subsequently analyzed by high-performance liquid chromatography (HPLC) and high-speed countercurrent chromatography (HSCCC). Additionally, fingerprint analysis of houttuynia cordata injection was performed by gas chromatography (GC) and chemometrics approaches, and related quality control for houttuynia cordata injection was further discussed and studied as well. The thesis mainly includes the following four aspects:In section 1, the basic principle, development and application of HSCCC and GC were reviewed. At the same time, the application to fingerprint in the study of traditional Chinese medicines was detailed introduced as well.In section 2, HPLC was introduced to modified the extraction method of Radix Angelicae, Radix Angelicae was extracted by petroleum ether, then extracted by ethyl acetate. The optimal solvent system of HSCCC for Radix Angelicae extracted by petroleum ether was n-hexane, ethyl acetate, methanol and water, with the volume ratio of 6:0.6:3:1. With the help of the optimal solvent system, four main components were well separated and purified, one of which was identified as ligustilide with the purity of 98.9%.In section 3, the optimal solvent system was selected by HPLC for Radix Angelicae extracted by ethyl acetate. The optimal solvent system was finally determined as n-hexane, ethyl acetate, methanol and water, with the volume ratio of 1:2:0.8:1. With the help of the optimal solvent system, two main components were well separated and purified, one of which was identified as ferulic acid with the purity of 99.8%.In section 4, the chemical components of the houttuynia cordata injection were qualitatively and quantitatively analyzed by fingerprint approaches derived from GC.The thesis provided the theoretical and experimental foundation for isolation and purification of effective components of Radix Angelicae. Furthermore, we also determined a set of effective analysis approaches for houttuynia cordata injection, including fingerprint analysis and qualitative and quantitative analysis of chemical components.