The Impact Of Pingfei Mixture Containing Serum On The Proliferation Of Human Lung Adenocarcinoma A549 Cells

This thesis includs two parts:a literature review and a experimental study.The progression of the TCM therapy of lung cancer was reviewed in this literature review, including five parts:etiology and pathogenesis, syndrome differentiation, the TCM treatment research, the syndrome differentiation and treatment of integrated traditional Chinese and western medicine, the mechanism of Chinese medical treatment for lung cancer. The literature review showed that integration of traditional and western medicine treatment of lung cancer emphasizes the syndrome differentiation, specimens consideration, integration of strengthening healthy energy and eliminating evil. And this kind of treatment obtained satisfied clinical effect on improving clinical symptoms, reducing chemoradiotherapy adverse reaction, enhancing the quality of life, prolonging the survival time.But part of mechanism of TCM therapy for cancer is still unknown. This would be likely to create difficulties on globalizing. This research observed the inhibitory action of the medicinal serum of the Pingfei mixture to the proliferation of the human lung adenocarcinoma cell line A549 through serum pharmacology methods.Purpose:This research discussed the inhibitory action of the medicinal serum of the Pingfei mixture to the proliferation of the human lung adenocarcinoma cell line A549, through serum pharmacology methods on the basis of previous studies.Methods:Serum containing Pingfei mixture was prepared by serologic pharmacology method. Pingfei mixture was administered orally to SD rats at doses of 20g/kg/d. The control group was 2ml/rat/d respectively twice a day from the 1st to 4th day. Blood was obtained from abdominal aorta aseptically at the 5th day 2 hours after the last morning administration and then the serum was separated. The proliferation of A549 after exposure to the serum was measured by CCK-8 assay.Results:The growth of A549 could be suppressed at the 48h after exposure to the serum from rats (P<0.05). The inhibition was enhanced over time. The results exists significant difference compared with the control group at the 72h and 96h (P<0.01) Conclusion:The Pingfei mixture could inhibit proliferation of A549 leading to anti-tumor effects. The work in this paper supplied basis for the study on mechanism of Pingfei mixture.