Twirling And Reducing Method On The Stress-induced Hypertension In Rats Hypothalamus Of Nos-1, Of Cgrp, Et-1 Gene Expression,

ObjectiveThe study aims at observing the effect on the gene and protein expression of NOS, CGRP, ET in hypothalamic of SIH rats treated by twirling reinforcing and reducing manipulation with Q-PCR and WB, investigating the difference on blood pressure and vascular endothelial factor expression of SIH rats between twirling reinforcing manipulation and reducing manipulation, and analyzing how twirling reinforcing-reducing manipulation bring high blood pressure down from vascular endothelial function controlled by central nervous system angle. It provides scientific bases for the validity and diversity on twirling reinforcing-reducing manipulation and experimental bases for the mechanism that acupuncture treats hypotension.Method60 clean male Wistar rats were divided into 5 groups such as controling group, modeling group, the acupuncture group, reinforcing manipulation group and reducing manipulation group. Electric shock and noise were applied to stimulate the rats into stress-induced hypertension model except controlling group. After modeling, the acupuncture group was treated by needling "tai chong" point without manipulation. Reinforcing manipulation group was treated by needling "tai chong" point with twirling reinforcing manipulation and reducing group was treated by needling "tai chong" point with twirling reducing manipulation. The treatments were operated once per day with a 7-day period. The systolic blood pressure (SBP) was measured in all the groups 1 day before modeling, the third, sixth, ninth, twelfth, fifteenth during modeling and the day after treatment. Then, the gene and protein contents of NOS, CGRP, ET in hypothalamic were detected by means of Q-PCR and WB.Result1 comparison of the SBP in groupsBefore modeling, there was no significant difference among groups (p>0.05). After modeling for 15 days, compared with the controlling group, the SBP of the other four groups was significantly higher (p<0.01). And there was no significant difference between the four modeled groups (p>0.05). After acupuncture treatment, the SBP of the acupuncture group and reducing manipulation group was lower than the one after modeling (p<0.01), which is not significantly difference from the one when before modeling (p>0.05). The SBP of the reinforcing manipulation group was lower than the one after modeling (p<0.01) and higher than the one before modeling (p<0.01).2 comparisons of the NOS-1 gene and protein expression in the hypothalamic in groupsCompared with controlling group, the NOS-1 mRNA expression of the modeling group, acupuncture group and reducing manipulation group was lower (p<0.05 or p<0.01). There was significant difference between reinforcing manipulation group and reducing manipulation group.Compared with controlling group, the NOS-1 expression in the hypothalamic of the modeling group, acupuncture group, reinforcing manipulation and reducing manipulation group was lower (p<0.01). And the one of the modeling group and acupuncture group was lower than reinforcing manipulation group and reducing manipulation group (p<0.01). There was significant difference between reinforcing manipulation group and reducing manipulation group (p<0.01).3 comparisons of the CGRP gene and protein expression in the hypothalamic in groupsThe CGRP mRNA expression in the hypothalamic was no significant difference among the groups (p>0.05).Compared with controlling group, the CGRP expression in the hypothalamic of the modeling group, acupuncture group, reinforcing manipulation and reducing manipulation group was higher (p<0.01). Compared with modeling group, the CGRP expression in the hypothalamic of acupuncture group, reinforcing manipulation and reducing manipulation group was higher (p<0.01). And there was significant difference among acupuncture group, reinforcing manipulation and reducing manipulation group (p<0.01).4 comparisons of the ET-1 gene and protein expression in the hypothalamic in groupsThe ET-1 mRNA expression in the hypothalamic was no significant difference among the groups (p>0.05).Compared with controlling group, the ET-1 expression in the hypothalamic of the modeling group, acupuncture group, reinforcing manipulation group and reducing manipulation group was lower (p<0.01). And the one of the modeling group and reinforcing manipulation group was lower than acupuncture group and reducing manipulation group (p<0.01).Conclusion1. The method can effectively establish the stress-induced hypertension model that Wistar rats are shocked by electric on foot and stimulated by noise. In the hypothalamic of SIH rats, the NOS-1 gene and protein expression could be reduced or restrained. There are no significant changes in CGRP and ET-1 mRNA. It may be related with the detection time. The expression of CGRP and ET-1 is decreased. They may play a compensatory effect to bring the SBP down.2. Needling "tai chong" point can effectively reduce the SBP of the SIH rats. In three treatments, the effect of twirling reducing manipulation is best, and twirling reinforcing manipulation is worst.3. The mechanism that the two manipulations bring the SBP down may be different. The approach that the twirling reducing manipulation bring the SBP down may be closely related to CGRP and there is no direct relationship with NOS-1 and ET-1. The approach that the twirling reinforcing manipulation brings the SBP down may be closely related to NOS, and there is a certain relationship with CGRP and ET-1.4. There are differences in the effects between the twirling reinforcing-reducing manipulations whose concept is reinforcing when turning left and reducing when turning right. And it is effective that turning left or right makes a distinction between twirling reinforcing manipulation and reducing manipulation.