| persimmon (Diospyros Kaki L.f), treated as homologous of food and medicine, contains a variety of biologically active ingredients, helps reduce blood pressure, soften blood vessels, increase coronary flow, Diminish inflammation and can improve cardiovascular function, but also promote the oxidation of ethanol in the blood, help the body's excretion of alcohol, reduce alcohol injury on the body, such as persimmon organic acids contribute to gastrointestinal digestion, increase appetite, and have a dry intestinal bleeding effect, it was important to research and development value. In this paper, persimmon as raw material, the separation of persimmon polysaccharides, purification, structural analysis, and In vitro antioxidant activity.The main conclusions of this article are as follows:1.This is a experiment studied on the extraction process of polysaccharide of persimmon used water extraction method. The influence on the extraction ratio of polysaccharide in persimmon such as solid-liquid ratio, extraction temperature and time was researched through the single-factor experiments and orthogonal test.The results show that the order of all influence factors are following:extraction temperature>solid-liquid ratio>extraction time, the optimum process are: solid-liquid ratio 1:10, extraction temperature 90℃, extraction time 4h. Under these conditions, the obtained polysaccharide content is 7.62%. The experiment studied on the ultrasonic extraction technics of polysaccharide of persimmon showed that the order of all influence factors are following: extracting temperature>extracting time> olid-liquid rati>ultrasonic extraction time, the optimum technics are:solid-liquid ratio 1:25, extracting temperature 80℃, ultrasonic extraction time 35min, extracting time5h., Under these conditions, the obtained polysaccharide content is 14.17%.2. The ethanol precipitation optimization of process are:4 times the volume of 95%ethanol, ethanol precipitation time 12h..3.Themethods for the effective protein removalof the crude polysaccharide from persimmon were studied. Deproteinization rate and polysaccharide loss ratewere used as the detecting index. Effectsofthree differentmethodson the protein removalby crude polysaccharide from persimmon were tested. Binding assay of papain-Sevag showed higher removalpercentage ofprotein and lower loss rate of polysaccharide.76.2%polysaccharides holding rate,61.2% protein removal rate.4. Comparison of the carbon and hydrogen peroxide on the color removal efficiency of polysaccharide persimmon, persimmon determine the best method for removal of polysaccharides and pigment conditions were:30% hydrogen peroxide:Persimmon polysaccharide solution (v/v)= 1:1, pH=10.0, temperature 60℃, time 1h, persimmon retention rate of 94% polysaccharides.5.Two kinds of homogeneous persimmon polysaccharides WPP1 and WPP2 were attained from the crude polysaccharide through cetyltrim-ethlammonium bromide(CTAB) and Sephadex G-100 gel filtration chromatography. The molecular weight of WPP1 and WPP2 were 2.05×105Da and 2.63x105Da by the determination of high performance gel permeation chromatography (HPGPC).Gas chromatography (GC) analysis showed that WPP1 was composed of rhamnose,.arabinose,glucose, D-galactose in molar ratios of 0.8831:0.6862:0.7022:1 and WPP2 was composed of arabinose, D-galactose in molar ratios of 0.8466:1.The glycosidic conformation of WPP1 was mostly a-linked glycosidic bond and both of WPP1 and WPP2 was characteristic of pyranose.6.On three kinds of polysaccharide (WPP, WPP1, WPP2) for in vitro antioxidant tests, the results show that:in the tested concentration range, three kinds of polysaccharide showed some scavenging hydroxyl free radical, DPPH radical, superoxide anion scavenging ability and the removal efficiency proportional to the concentration of polysaccharides; at the same concentration conditions, WPP's ability to be significantly higher than that of clear WPP1 and WPP2. |
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