| Purpose:Through the observation Guizhi Fuzi decoction of lateral of rheumatoid arthritis, discusses the influence of rats of aconite guizhi decoction of rheumatoid arthritis treatment mechanism for treatise on febrile disease, the clinical application of hydrosphere party lay a theoretical foundation.Material and method:O-level health SD rats 40 only, male and female, random evenly divided into four groups: blank group (group A), model group (group B), cassia twig aconite soup group (group C), tripterygium wilfordii group (D group), each group of 10 only. In addition to A group of outside B, C, D three rats, sterile conditions only right rear foot pad skin each injection in eph's complete adjuvant 0.1 ml preparation rheumatoid arthritis model. For the first seven days, build mode to group C aconite Guizhi Fuzi decoction fluid, according to another 1.5 ml/is 100 gold weight/give only medicine, A, B two groups were given equal sterile saline, group D give tripterygium wilfordii solution, according to how glucoside 1mg/l00g weight/only to medicine. In the first twenty-eight days, take and the indicators testing left ankle joint, making pathological, using light microscopy.Results:1. Animal state: Model group, Guizhi Fuzi decoction group, tripterygium wilfordii group rats after gangrenous toe in building mode gradually swelling, one day later, achieve the peak, the swelling lasts for 3 days and start to decline untill the eight day, then on the fourteen day achieve the peak; the local skin redness and swelling, and have obvious dodge symptoms, food volume also have different degrees of reduction.From the ten day, the left limbs is obviously swollen, ears and tails of mouse appear phlegmasia nodule and erythema, secondary response with hyperremia nose mucous membrane; the secondary response and the swelling of left limbs of Guzhi Fuzi decoction group are not obvious.2.Pathological observation: In model group, mononuclear cells infiltration of cells is frequently seen, synovial hyperplasia of the basic normal, arrange the phenomena is disorder, and large necrotic tissue surface coarse. With a small amount of aconite Guizhi Fuzi decoction group lymphocytes and mononuclear cells infiltration of cells, synovial hyperplasia of the basic normal, not arrange the phenomena and large necrotic tissue surface slightly coarse; Tripterygium wilfordii group also has a few lymphocytes and mononuclear cells infiltration, visible synovial hyperplasia of membrane, were organization mild slightly rough. Explain Guizhi Fuzi aconite soup and tripterygium wilfordii treatment rheumatoid arthritis, and curative effect good curative effect quite.3. Serum IL - to the influence of six concertration: Blank group and the model group, and group compared aconite Guizhi Fuzi decoction, a statistically significant (P < 0.01), but the blank group and tripterygium wilfordii group was not statistically significant (P > 0.05). Guizhi Fuzi decoction group compared with tripterygium wilfordii group though a statistically significant (P < 0.05), but did not differ significantly. Guizhi aconite soup group and tripterygium wilfordii group compared with model group was statistically significant (P < 0.01).4. On serum TNF alpha of concentration: Model group and blank group, cassia twig aconite soup group, tripterygium wilfordii group, a statistically significant compared (P < 0.01), blank group and tripterygium wilfordii groups and Guizhi Fuzi decoction of lateral group compared also have statistically significant (P < 0.01), but tripterygium wilfordii group and cassia twig of aconite soup than was not statistically significant (P < 0.05).5. The transformation of lymphocyte impact: Model group and blank group, cassia twig aconite soup group, tripterygium wilfordii group, a statistically significant compared (P < 0.01), blank group and tripterygium wilfordii groups and Guizhi Fuzi decoction of lateral group compared also have statistically significant (P < 0.01), but tripterygium wilfordii group and cassia twig of aconite soup than was not statistically significant (P > 0.05).6. On the influence of serum hemolysin value: Model group and blank group, cassia twig aconite soup group, tripterygium wilfordii group, a statistically significant compared (P < 0.01), blank group and tripterygium wilfordii groups and Guizhi Fuzi decoction of lateral group compared also have statistically significant (P < 0.01), but tripterygium wilfordii group and cassia twig of aconite soup than was not statistically significant (P > 0.05).Conclusion:1. Guizhi Fuzi decoction in the treatment of lateral rheumatoid arthritis is effective, Its mechanism is probably decreasing serum mediators of inflammation, inhibiting the accentuation of cells and humoral immunity, to relieve the body inflammatory reaction, improve the microcirculation and the immunizing regulation.2. The role of Guizhi Fuzi decoction is to balance yin and yang. |
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