Guizhi Aconite Decoction Treatment Of Adjuvant Arthritis In Rats

Objectives:This experiment is intended to evaluate the safety, effetiveness of treatment of rheumatoid arthritis by Guizhi Fuzi Decoction to for effective treatment of Chinese medicine of rheumatoid arthritis prescriptions, as well as "Treatise on Febrile Diseases" by the side of the clinical applied research has a good foundation.Materials and methods:Totally 48 heathy Wistar rats were randomly divided into 4 groups: Normal control group, model group, Leigongteng group, Guizhi Fuzi Decoction group, which all have 12 rats, excepting normal control group, other 36 rats were made into rheumatoid arthritis models by subcutaneous injection of complete Freund Adjuvant. After made into rheumatoid arthritis models 12 days, rats in the normal control group drank water freely; rats in the model group were perfused with 1. 5ml of distilled water once a day; rats in the Leigongteng group were poured internally with 1mg/ml Leigongteng solution; rats in the Guizhi Fuzi Decoction group were poured internally with Guizhi Fuzi Decoction solution. Enzyme linked immunosorbent assay (ELISA) was adopted to detect the level of IL—1β" and PGE₂ in the serum of rats on the 21th day after administration.Results:1. The content of IL-1β: For the content of IL-1βin the serum of rats, between model group and Leigongteng group, Guizhi Fuzi Decoction group, normal control group, there was significant difference in statistics. It demonstrates that the content of IL-1βin the serum of rats in model group is much more than Leigongteng group, Guizhi Fuzi Decoction group and normal control group. Between Guizhi Fuzi Decoction group and Leigongteng group there was no significant difference in statistics. It implies Guizhi Fuzi Decoction group as same content of IL-1βas Leigongteng group.2. The content of SOD: For the content of SOD in the serum of rats, between model group and Leigongteng group, Guizhi Fuzi Decoction group, normal control group, there was significant difference in statistics. It demonstrates that the content of SOD in the serum of rats in model group is much less than Leigongteng group, Guizhi Fuzi Decoction group and normal control group. Between Guizhi Fuzi Decoction group and Leigongteng group there was significant difference in statistics. It implies the content of SOD in the serum of rats in Guizhi Fuzi Decoction group is much more than Leigongteng group.3. Pathological observation: As compared with model group, Guizhi Fuzi Decoction could produce the decreases in hyperplasia of synovial cells, congestion and dropsy of synovium, hyperplasia of blood vessel, infiltration of inflammatory cell, and formation of pannus. The smooth surface of articular cartilage and the normal subcartilaginous bone were seen in groups of Guizhi Fuzi Decoction and Leigongteng.Conclusion:Guizhi Fuzi Decoction can control the activities of Inflammatory mediators like the IL—1βand save the content of SOD in the serum of adjuvant-induced arthritis rats, it is effective to rheumatoid arthritis.