Zuojinwan Potency Of The Experimental Study

The paper repored the study on the testing method of the nature of Chinese herb, and the nature of Zuo Jin Pill (ZIP), especially about its effects on the indexes of heat syndrome, and cold syndrome were also studied. In Test I the sthenic heat syndrome model of rats were established by administering the decoction of Radix Codonopsis and Radix Astragali orally so as to study ZJP - s effects on it. Some experiments were carried out, for instance, the changes of the hepatocyte ultrastructures were observed with transmission electron microscope of Model JEM-I200EX, the concentrations of T3, rT3, T4, TSH, cytokine IL-6, IL-8 and F in rat sera were examined by the method of radioimmunoassay, the concentrations of NE, DA in sera were measured with spectrofluorometer, the concentration of I 7-OHCS in sera, the activities of Na+-K+-ATP enzyme and Ca2+-Mg2+-ATP enzyme in erythrocyte membranes were measured with spectrophotometer of Model 722, and the changes of temperature and weight were also examined. The results showed that the hepatoeyte ultrastructures of rat in heat syndrome restore to normal nearly in Group 6 1 and Group 2 :i, and partially in Group 1 :1; the anal temperatures were decreased significantly in Group 6 1; the concentrations of NE, DA, F, TSH, IL-6, IL-8, adrenal I 7-OHCS in rat sera and the activities of Na -K4 -ATP enzyme and Ca2+-Mg2+ -AlP enzyme in erythrocyte membranes were decreased significantly in Group 61 and Group 21; the concentrations of T3, T4 in sera were decreased, that of rT3 was increased in Group 6 1; the concentration of T4 in sera was also decreased in Group 2 1 and that of IL-8 in sera was decreased in Group 1 1. In Test II the cold syndrome model of rats were established by administering the decoction of Radix et Rhizoma Rhei, Rhizoma Coptidis, Radix Scutellariae orally, then the rat were administered ZJP orally, and the concentrations of NE, DA, 1 7-OHCS in rat sera, the activity of Na+-K+-ATP enzyme in erythrocyte membranes were measured, and the changes of weight and temperature were examined. The results showed that the anal temperatures were all decreased in Group 6 1 and Group 2 1; the concentrations of NE, adrenal 17-01-IC S in sera and the activity of Na + -K + -AlP enzyme in erythrocyte membranes were decreased significantly; the concentrations of DA in sera were all decreased significantly in Group 6 1, Group 2 1 and Group 1 1, and the activity of Na+-K+-ATP enzyme in erythrocyte membranes was also decreased significantly in Group 2 1. The results showed that ZJP of Group 6 : I can mitigate heat syndrome significantly or aggravate cold syndrome significantly, Group 2 1 can also mitigate heat syndrome significantly or aggravate cold syndrome, but the effects of Group 6 1 and Group 2 1 on some indexes were different; the effects of Group 1: 1 on certain index indicated that it had the functions of mitigate heat syndrome or aggravate cold syndrome, but the effects on the majority of indexes indicated its functions were not distinct. So ZJP is cold in nature when the doses of Rhizoma Coptidis and Fructus Evodiae are in the ratio of 6 : 1 and 2 :1, but when the doses of Rhizoma Coptidis and Fructus Evodiae are in the ratio of 6 1 the cold in nature of ZJP is greater than that of ZJP when in the ratio of 2 :1, and when in the ratio of 1 I ZJP is mild in nature (slightly mild to cold in nature).