Microdialysis Glucose Detection System

Many researches have been reported to show that the method of co-immobilizing a hydrogen peroxide-producing oxidase with the peroxidase results the responsibility of an amperometric biosensor essentially being improved. A developed microdialysis system for continuous glucose measurement using this effect by co-immoblilizing is overviewed. It consists of a microdialysis probe, an interval driven pump, a micro enzyme reactor and a waste collection. The interval time and the flow rate of the pump is performed. Mainly in the enzyme reactor, a special micro sensor configured with solid co-enzyme is reported. It is based on co-immobilizing straticulately glucose oxidase (GOD) with the peroxidase on the surface of the silicon-based microelectrode using the tetranethoxysilane (TMOS) sol-gel thin film technique. Some different schemes of immobilizing enzyme with sol-gel film are performed. Above all a graduated ("Sandwich") immobilizing method is preferred. The characters of the co-enzyme (and GOx only) sensors modified with SiO2 gels is determined. Many experiments have been evaluated to compare them. An interdigital microelectrode is designed for micromation. Furthermore, a new bi-spire microelectrode is presented, and its character is discussed.