The experiment of Cinobufacifli--induced aPoptosis. m' ln ffe-60 cells. Abstract -Objective: To observe the Cinobufacini -induced apoptosis inHL-60 cell.s and exP1ore its mechanism to provide experimentalevidence fOr treatment of leukemia with Cindbufacini.MethQds: Cellular groWth curve .and MTT assay were undertakento observe the growth inhibition of m-60 cells expo5ed tocinobufacini .The Cinobufacini --induced apoptosis in HI-60 cellswas detected by the cell morphoIogical observation,flow cytometryand Annerin V /PI double-. labeling and mitochondriatransmembrane Potefltial Was measured by Whodaxnine l23staining.ResultIs:l' Cinobufactal obviously iMibit the pro1iferation ofm-60 cel1s in atdrie-dependent and dose-dependent manner at -tthe range ofconsiStency from 0.0625 to 0.5 P g/nil.2. There aPpears the chaxacter of aPoptotic ceIls in m-60 cellsfOllowing the eaposure to cinobufacthe eg. cellu1ar atrophychIomatin clustering,karyoldasis.3..The results detected by flow cytometry and Annexin V RI double labeling quantative methods showed that obvious dose-dependent manner exists in the Cinobufacini-induced apoptosis in HL-60 cells at the range of consistency from 0.125 to 0.5 ~?glml.4..HL-60 cells treated with Cinobufacini showed both apoptosis and mitochondria transmembrane potential collapse.Conclusions:Cinobufaeini could inhibit the proliferation and induce apoptosis in HL-60 cells in vitro ,accompanied by whichihe appearance of mitochondria transmembrane potential collapse imply that the effect of Cinobufacini might be related with the destruction of mitochondria function.Being a. hypotoxicity antineoplastic, Cinobufacini has been attemptted to treat neoplasm whose effect need further exploring in the treatment of leukemia. |