Nano-carbon Preparation

Active carbon has strong capacity of adsorption. The common active carbon in market is only used to remove colors,adsorb heat source and delete tastes ect. In this article,microparticle ball mill is used as grinder equipment,which produced nanoparticles of active carbon( 1 OOnm in diameter). Furthermore ,the technology of preparation,physicochemical properties,the stability of prepared suspensions and preliminary clinical application are also studied in detail.At the same time,5-FU is used as model drug(i.e.5-FU-CH dosage form). This article has investigated the in vitro release of the drug,active toxity,preliminary pharmacokinetics and curative effects ect.,with the traditional form of 5-FU梥olution of 5-FU (i.e.5-FU-H20) as reference. During the research of the preparation of nano-carbon particles,the effect of factors including the time and rate of grinding,ratio of large steel balls to small ones and the ratio of amount of active carbon to that of water on the smashing were investigated by single-factor study.Formulation was optimized through orthogonal design experiment.Some physicochemical property of nanoparticles of active carbon such as diameters,specific surface area,buck density,angle of repose and hygrosopicity were studied. Meanwhile,we have observed the effect 3 ABSTRACT of different suspension aid on suspensions,made sure that PVP should be the suspension aid and detected the effects of different process of preparing suspensions,different batches,various concentrations of PVP and temperatures on the stability of suspensions by freezing-heating circulation test,observing samples under combient temperature and rheometry test. The result shows that suspensions were more stable with narrower diameter of nano-carbon particle and larger specific surface area,which was applied preliminarily to direct the clearance of lymph nodes as indicator during the clinical operations.The effects are satisfactory. Nano-carbon suspension containing 5-FU was prepared.We investigated how the three factors such as dose of 5-FU,concentrations of nano-carbon and PVP influenced the release of the formulation and gained the optimal formulation by orthogonal design experiment.Compared with 5-FU-H20,the active toxity of 5-FU-CH was examined preliminarily. The LD50 were 216mg/kg and 600mg/kg,respectively. The method of HPLC was emphasizedly employed to compare the pharmacokinetics of 5-FU-CH and 5-FU-H20 formulation after their administration in the obdominal cavity of naked mice suffering from ascites cancer.The results demonstrated that AUC of 5-FU-CH formulation was smaller than that of 5-FU-H20,proving that drug concentration in prepheral plasma was more lower than that of 5-FU-H20 formulation. All of this indicated that this formulation was more detained locally on the focus site of obdominal cavity than 5-FU-H20 formulation. During the experiment of curative effect,we still used the naked mice suffering from ascites cancer as test animals and compared existing days of control group,5-FU-CH group and 5-FU-H20 group. The result reflected that the existing days of naked mice were significantly 4 ABSTRACT delayed.