Effects Of Actived Carbon Nano-particles On The Anti-cancer Action Of DDP

Objective: To observe the effect of actived carbon nano-particles (ACN) and carbon aerogels (CAG) on the anti-cancer action of cisplatin and on the immune function of the mouse. Methods: MTT and Flat plate clone formation were adopted to evaluate the effect of ACN and CAG on the inhibitory action of cisplatin on the growth of BGC823 cells; The effect of ACN and CAG on the inhibitory action of cisplatin on tumor growth was evaluated by constructing S180 carcinoma, H22 hepatoma, Lewis lung cancer and B16 melanoma transplantation tumor model. 3H-TdR incorporate method was used to observe the effect of ACN and CAG on spleen cell proliferation. ELISA was used to evaluate the effect of ACN and CAG on the level of IgG in the culture supernatant of spleen cell. Flow cytometry was used to evaluate the subpopulation of spleen cell. Results: (1) ACN alone had no effect on the clone formation of BGC823 cells, but it had an obvious synergistic effect on the inhibitory action of cisplatin on the clone formation of BGC823 cells. (2) Acording to a series of experiments, We finally selected the experiment condition. The dosage of cisplatin was 2 mg/kg and ACN was 5 mg/kg. (3) Cisplatin (2 mg/kg) had an obvious inhibitory effect on the growth of the tumor. ACN (5 mg/kg) had an obvious synergistic effect on the inhibitory action of cisplatin on the growth of S180 carcinoma and H22 hepatoma, but weakend the inhibitory action of cisplatin on the growth of Lewis lung cancer and B16 melanoma. We considered the solvent of ACN-the blood lysate may had an effect on the anti-cancer action of cisplatin, so we researched the effect of the blood lysate on the anti-cancer action of cisplatin and on the immune function of the mouse. (4) The blood lysate had a tendency of weaking the inhibitory action of cisplatin on the growth of S180 carcinoma and H22 hepatoma and obviously weaked the inhibitory action of cisplatin on the growth of Lewis lung cancer; The blood lysate obviously inhibited T cell proliferation and B cell proliferation. The blood lysate also had an obvious inhibitory action on the level of IgG in the culture supernatant of spleen cell. It also decreased the CD4+ cell percentage, heightened the CD8+ cell percentage, thereby the CD4+/CD8+ ratio obviously decreased. Therefore the blood lysate may promote the growth of the tumor according to its inhibitory action on the immune function. (5) ACN obviously enhanced T cell proliferation and obviously raised the level of IgG in the culture supernatant of spleen cell. It also obviously heightened the CD4+ cell percentage, decreased the CD8+ cell percentage, thereby the CD4+/CD8+ ratio obviously heightened. (6) CAG-301 had a tendency of strengthening the inhibitory action of cisplatin on the growth of S180 carcinoma. It also enhanced B cell proliferation and obviously raised the level of IgG in the culture supernatant of spleen cell. CAG-301 (1-50μg/mL) alone had an obviously inhibitory action on the growth of BGC823 cells. CAG-301 (25-50μg/mL) had an obvious synergistic effect on the inhibitory action of cisplatin on the growth of BGC823 cells. Conclusion: ACN and CAG can enhance the anti-cancer action of cisplatin and can also improve the immune function of the mouse.