Human Herpesvirus 8 Dna In Kaposi's Sarcoma In Xinjiang Organization Fluorescence In Situ Pcr Assay,

Objective Although Kaposi抯 sarcoma (KS) initially was described over a centuly ago, its biology remains entigamatic and conflicting. Several infections agents, including herpesvirus-like particles, had been suggested as possible candidates for the development of KS, and a new herpesvirus, human herpesvirus 8 (HHV-8), was recently identified in the vast majority of KS lesions, irrespective of their association with human imrnunodeficiency virus (HIV) infection. We undertook this study to screen for and incalize the presence of HHV-8 in KS in Xinjiang. Methods We performed fluorescence in situ polymerase chain reaction to detect I-IHV-8 DNA. A total of 40 parafiln-embeded specimens were studied, including 20 KS lesions (12 nodular lesions, 6 plaque lesions and 2 patch lesions) and 20 non-KS lesions (18 dermatofibroma and 2 hemaiigioma). Results Among the 20 KS lesions, the HHV-8 DNA was detected in 17 lesions (the positive rates were 85%) and n the other non-KS lesions, HHV-8 DNA was not found (p<0.00 1). Positive signals for I-IHV-8 were demonsttated in the nuclei of spindle-shapped turner cells and enothelial cells. In addition. po~ftive signals were also presented in the epidermal keratinocytes, however, the signal was weaker than in the spindle-shapped turner cells and the enothelial cells.Conclusions The result of this study suggested that HHV-8 may not widespread and had a certain causative role in the pathogenesis for Xinjiang KS.