The Antagonistic Effect Of Zinc, Iron, Vitamin C, Manganese Cell Immunotoxicity Observed

Effects of Zinc,lron and L-ascorbic Acid on Antagonism tocen -mediated Immunotoxicity Exposcd to ManganescSchool or Pubnc Htw Xinhag Mwhtal bohayyrumctoPostgraduate Liu FengjuanTutors Huang Xianyi Yao HaiytngAbstractlObjective The effects of zinc,iron and L-ascorbic acid on cellmediated inmiunc~toxicity after exposure to manganese were slxidied.Methods Lymphocytes from peripheral blood mononuclear were cultured in RPMI1 640 basal medium with supplementation of zinc,iron and L-ascorbic acid when exposed to manganese. Lymphocyte proliferation and natural killer cell activity were assessed by meanes of MTT assay,and the concentration of IL-2 by ELISA.And orthogonal design were used to analyse data.Results Lymphocyte proliferation,NK activity and the concentration of IL-2 decreased when the concentration and the duration of exposure to manganese increased. Zinc was the most important factor in the antagonism of them to cellmediated immunotoxicity exposed to manganese. The concentration of IL-2 greatly increased when the concentration of zinc went up, and Fe/Zn=0.In addition,there was a tendency that NK activity went up as the concentration of zinc and antagonistic duration increased.Conclusi~n Appropriate concentration of zinc could antagonize cell-mediated immunotoxicity induced by manganese.