| Objective: To study the protective effect and mechanism of PUC (Compound of Phyllanthus urinaria,PUC) on experimental liver injury in mice. Methods: The models of CCL4-induced acute and chronic liver injury in mice were used in the experiment. The experimental mice were divided into 6 groups: untreated , CCL4 control , western medicine , PUC high dose (PUCH),PUC middle dose (PUCM) and PUC small dose (PUCL) group(containing crude herbs 60,30,15g/kg weight respectively). Biophenyldicarboxylate(BPD) and colchicines were used as western medicines in the acute and chronic liver injury experiments respectively. Alanine transaminase(ALT),aspartate aminotransferase(AST),superoxide dismutase(SOD),malondialdehyde(MDA),total protein(TP) and albumin(ALB) in serum and Hydroxyproline(Hyp) in liver were measured. We also observed the changes of liver histopathology and the mice body weights. Results: PUC can decrease the contents of ALT,AST and MDA in serum and the level of Hyp in liver and increase the contents of TP,ALB and SOD remarkably; Besides, PUCH has stronger effects on lowing the content of MDA and heightening SOD than BPD and colchicines; In the acute liver injury experiment, PUCH and PUCM have a similar effect on decreasing ALT and AST to BPD; while PUCH has stronger action in lowering the activities of ALT and AST and the level of Hyp than colchicines in chronic liver injury experiment; And the dose-dependent relation among the PUC groups is seen; Histopathologic examination shows that PUC can relieve liver injury and hepatic fibrosis in mice induced by CCL4; The body weights of mice in six groups are increased after the chronic experiment finished, but the CCL4 model group's are increased lightly; The mice weights of PUCH group are near to the untreated group's. Conclusion: PUC has significant protective effect on liver injury in mice induced by CCL4, possibly due to its anti-lipid peroxidation capability, inhibition of collagen synthesis and facilitation of protein synthesis. |
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