| ObjectiveAsarurn is a kind of Chinese herbs in common use, and its clinical application contains more than 2000 years. Because it is poisonous, there is a popular saying "No Asarum can be used over one qian" (qian, a measure unit of weight). The Pharmacopeia (abbr.Ph) of the People's Republic of China (1st part, published in 2000) (to call for short below: China abbr.Ph) demonstrably prescript Asarum' dosage is 1 ~ 3g. However, the dosage China abbr.Ph prescripted cannot be always abided by clinicians. There are always many cases of curing types of disease with heavy dosage of Asarum, with not any noxious or side effects but with the satisfactory results.Now the reason lies in no more acknowledge with Asarum' virulence mechanisms. Because of this, through literature and experimental research, my scientific research topic aims at working out it. At the same time, the topic provide clinical application reference for the safe and efficient use of Asarum.Methods1. Literature investigation: After consulting much literature on it issued in the recent 30 years, and systemicly summing up, analyzing and research on the relative factors of Asarum toxicity such as variety, herborizing, making, usage, dosage, toxicity substance.2.Experimental researches: (1) Acute virulence experiment: The 200 experimental mices were divided into 2 groups in equality, namely: the Asarum roots powder group and the whole Asarum powder group. The chief aim is to observe medicated mices' toxic reaction and death stat, and to calculate each other' 50% lethal dose (LD50). (2)Histopathology research: We draw forthwith the heart constitution, liver constitution, lung and kidney constitution from the dead mices (including of the decollated mices lived after 14 days), and use formaldehyde fossilize them, make filletings, HE Stain, and then observe by light microscope to research into the Asarum' influence on the importanter viscera. (3) Experimental rabbits' respiratory movement and neuroelectricit research: Taking the research records of comparison and analysis of the Asarum medicated and unmedicated rabbits' phrenic nerve activities and Changes with the BL - 410 organism function experimental syste.Results1 .Literature investigation indicates: Safrole, a kind volatile oil of Asarum is the toxicity component. And so its toxicity degree has close relation to the volatile oil content. The application of Asarum now is much different from ancient. For instance, the ancients look on Asarum' roots as officinal locus, but modernist do the whole Asarum; Asarum was grinded powder and used solely in ancient times but isdecocted into soup in compound in modern time. And so, the acquisition season and how to make vary with officinal locus vicissitudes. All these factors are directly bound up with the the volatile oil content. Because of it, its toxicity degree and dosge differ in the two eras.2.Acute virulence experiment shows: It is determined that tHe LDso of the Asarum roots powder and the whole Asarum powder for mice in stomach administration is 6.5229g/kg and 11.7052g/kg respectively. It's clearly that he Asarum roots powder is more poisonous than the whole Asarum powder.3.Histopathology and respiratory movement research manifests: Heavy dosage of Asarum has toxic effect on lung, liver and kidney of mices in different degree. Among the three visceras, the lung constitution was damaged to the worst degree. The main lesion of lung constitution are as follows: There are congestion and bleeding in aniso-degree in it. It can be found leucocyte adhesiveness retention, emphraxis blood vessel in aniso-degree on alveolar wall blood capillary. More seriously, leucocyte mass or alveolar wall Increase thickly because of blood capillary infiltration.4.Experimental rabbits' neuroelectricit research evinces:Under administration, experimental rabbits' respiratory movement experience two stages: Respiration excite initially and get be restrained in anaphase. Correspondingly, breath's range and respiratory frequency and the amplitude of vibrati... |
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