| As a crystallization of wisdom of the Chinese Nation, Chinese Traditional Medicine Compound have an advantage in treatment of disease. Their anti-tumor activity is recognized by many people in the world. Finding new type anti-tumor drug from compound is an important way to exploit dominance of rich drug plant resource of china..Formation and growth of tumor depend on nourishment which supplied by angiogenesis. If the formation and growth of angiogenesis around tumor is controlled, the tumor growth can be controlled until its disappear. It was proved that the growth of angiogenesis is dependent on Methionine aminopetidase n, one kind of enzyme, which is shorted by MetAP2.If MetAP2 is controlled effectively, the growth of tumor then can be controlled. The Matter that can control MetAP2 will probably be a new type drug of anti-tumor.In this essay, a new type anti-tumor activity component of Dioscorea bulbifura L was studied and MetAP2 was made as a target to determinate anti-tumor activity. Dioscorea bulbifera L is one kind of anti-tumor Chinese Traditional Medicine in common use and it was proved to be of high curative effect during the long run.The ethanol extraction of Dioscorea bulbifera L is crudely separated by column chromatography and then the crude separation component was subdivided by centrifugal thin layer chromatography. All components are carried through activity assay according to MetAP2.Some components with high anti-tumor activity are found out from Dioscorea bulbifera L. Their structure are analyzed by GC-MS. They are Hexadecanoic acid, Octadecanoic acid and 9,12-Octadecadienoic acid. This fraction's activity is dependent on cooperation of components. On the other way, two kind of cholesterol are found out in the first time. They are Cholest-5-en-3-ol and Gama-ergostenol.These achievements offer strong theoretical foundation for development of new type anti-tumor drugs and popularization application of Dioscorea bulbifera L. |
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