| ObjectivesIt is a valid method characteristic of traditional Chinese medicine to treat the osteoarthritis in the therapy of plastering on the knee with Gu Tong Gao (GTG). This lesson is to understand the changes of the pathology and the content of the hexosamine, with the observation of image and super microstructurc of articular cartilage and the content of the hexosamine, and studying the mechanism that how does plastering on the knee with Gu Tong Gao (GTG) treat the osteoarthritis.Methods1. Grouping32 rabbits were randomly divided into 4 groups, each having 8. The normal group docs not make any handling, the sham-operation group, the THGT (tian he gu tong) group, the GTG (gu tong gao) treated group's skin of the middle right groin was cut open by right angle, sewing up after separating and ligaling the vein of thigh in right groin.2. Treatment methodThe GTG group and the THGT group had been applied plasters on for 3 weeks after the rabbits were remodeled 8 weeks later.3. Observation to the pathology of the articular cartilageObserves in the naked eye first each domestic rabbit articular cartilage surface circumstance, then will shear parts of articular cartilage specimen that take difference according to specimen system after having the method establishment, proceed the option mirror with give or get an electric microscope observe and take relative radiograph.4. Measurement to the content of the hexosamineMeasure to the content of the hexosamine of the rest specimen of the articular cartilage according to the standard curve calculation method. Results1. On the content of the hexosamine, the articular cartilage of the normal group was not changed, the articular cartilage of the model group was lower seriously, and the articular cartilage of GTG treated group's was changed less seriously than the one of model group.2. By the electron microscope, option and eyes, the articular cartilage of the normal group was not broken, the articular cartilage of the model group was broken seriously, the remodeled articular cartilage were break seriously, surface layer cartilage contain more cracks , peeling off formation defection and damage area, the cartilage cell arranges the mess, four layer constructions distinguish not easily, the tide line is not complete much, the rough endoplasmic reticulum degranulate, the number of the mitochondria reduced, necrosis cell broken bits therefore, cell nucleus shape anomaly, the chromatin concentrated, spread in the nucleus . and the articular cartilage of GTG treated group's was broken less seriously than the one ofmodel group, articular cartilage surface contain shallow cracks , the cartilage cell arranges tidy, report the pillar form, the tide mark line is complete, therefore more not mature cartilage cell, the rough endoplasmic rcticulum expansion pond, the golgi complex extended, therefore the cartilage cell secretes the gum original, and appear the more egg white many sugar grain, the stability that maintain the cartilage cell the membrane , promote the cartilage cell propagate to combine to urge to have a great achievement can of the cell machine increases to living, hinting the cartilage cell resumed the normal metabolism the level. Conclusion'The plastering on the knee with Gu Tong Gao has the clear and definite treatment result to the articular cartilage of the osteoarthritis model domestic rabbit, backing to change to prevent the function of the articular cartilage. Promoting the cartilage cell propagates to combine the cell machine that urge has a great achievement and can increase to living, improving the cartilaginous nourishment in joint circumstance, recovering the cartilaginous and normal physics and chemistry in joint environment to the best, the cartilage cell resumed the normal metabolism level, backing to change to alleviate the function cartilaginous to the joint. |
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