Baicalin Hepatic Fibrosis In Vitro Studies

Objective:This study was amended the method of isolating and culturing rat hepatic stellate cells.Methods: The rat hepatic stellate cells were isolated with collagena-se recirculating perfusion and purified by density gradient centrifugation.Resultesrln this way,the output of the hepatic stellate cells amounted to 1-3 X 107 cells per liver. The purity of the cells was above 91% . The viability of the cells was more than 95%.Conclusion:This is an economical and steady way of isolating and culturing rat hepatic stellate cells.