Astragalus Injection Anti-liver Fibrosis In Rats

Aims : To improve the method of isolating and culturing rat hepatic stellate cells. Methods: The rat hepatic stellate cells were isolated with collagenase recirculating perfusion and purified with density gradient centrifugation. All cells were cultured with DMEM medium supplied fetal calf serum and were passaged.Resultes: In this way, the output of the hepatic stellate cells amounted to 2-5×107 cells per liver. The purity of the cells was above 98%. The viability of the cells was more than 90%. Conclusions: This is an economical , high output and steady way of isolating and culturing rat hepatic stellate cells.