Leech Extract On Cultured Bovine Lens Epithelial Cells Of The Experimental Study

Objective To examine the effects of extracting solution of Hirudo on the proliferation, adhesion and adherence to intraocular lens(IOL) of bovine lens epithelial cells(LEC) cultivated in vitro, for evaluating the possibility of Hirudo on the prevention of after cataract.Methods 1. Bovine lens anterior capsule was cultured in vitro with explant culture method, and the cultured cells were identified by morphological examination and the characteristic lens-like spherules of LEC cultured in vitro.2. Five kinds of conventional extracting solution of Hirudo (alcohol and water extracting, alcohol refluxing, water extracting, water extracting-alcohol precipitating, percolation filtration) were used in this study. The passage LEC was incubated for 48h, then exposed the cells to different extracting solution in different concentrations for 24h and 72h. MTT method was used to detect the OD values and ID50 was calculated.3. The passage LEC and different extracting solution in high dosage(ID50) and low dosage(l/10ID50) were placed and cultured for 24h to investigate the inhibitive rate of cell adherence.4. LEC was incubated with IOL in high and low dosage of different extracting solution for 24h to investigate the inhibitive rate of cell adherence on the surface of PMMA IOL.Results The ID50 of these extracting solution exposed to LEC for 24h and 72h were between 20.56mg/ml to 68.78mg/ml, and inhibition of water extracting-alcohol precipitating solution and percolation filtration solution was more strongly(P<0.05); Compared with the control group, there wassignificant inhibition to LEC on cell adhesion and adherence on IOL of alcohol and water extracting solution, water extracting-alcohol precipitating solution and percolation filtration solution ( P<0.01 or P<0.05).Conclusion Five kinds of conventional extracting solution of Hirudo all can inhibit the proliferation of LEC cultivated in vitro, and inhibition of water extracting-alcohol precipitating solution and percolation filtration solution was more strongly among them. There was significant inhibition to LEC on cell adhesion and adherence on IOL of alcohol and water extracting solution, water extracting-alcohol precipitating solution and percolation filtration solution. Water extracting-alcohol precipitating solution and percolation filtration solution of Hirudo can significantly inhibit the proliferation, adhesion and adherence to IOL of LEC cultivated in vitro at the same time. This study gives some experiment foundation for extracting solution of Hirudo to prevent after cataract.