Toxic Components, Including The Aristolochia Medicine (manshuriensis)

Many Chinese-herbs come from the Aristolochiae plants, such as Fructus Aristolchia (FA) A.manshuriensis Kom.(AMK), A.debilis Sieb.et Zucc (ADS)etc. However, in the recent, more and more people have paid attention to the ill-effect of the induced by Aristolochinc acid (AA) around the world. In our study, we analyzed the quantity of the AA in the AMK. In addition, we analyzed the effect of the quantity of the AA in the AMK when making up with Radix et Rhizoma Rhei(RR) or Rhizoma Ligustici Chuanxiong(RLC). We have studied the structure-activity relationships of the AA with the molecular imitate techniques. We modified the structure of the AA, and made a comparison between the AA and modified-AA(M-AA) in the nephrotoxicity of the rat. The result of experiments showed as below:1.The AA contents of AMK was 5.6897mg/g by the HPLC. When the AMK made up with the RR the AA conrents can be reduced to 34.2%, and the RLC to 4.1%.2.The total AA contents of AMK was 15.1mg/g by the ultraviolet spectrophotometry. We used the iron as the reductive agent to reduce AA.3.We made a comparison between the AA and M-AA in the nephrotoxicity of the rat. The result shows: the M-AA can relieve nephrotoxicity of the rat, but it is not markable.