Experimental Study On Rabbit Blood-brain Barrier For Acute Cerebral Infarction. Electric

ObjectveIt is a valid method to treat the acute cerebral Infarction in acupuncture. This lesson is to understand the changes of the acute cerebral infarction rabbits' blood-brain barrier after being prinked ,with the observation of image and super microstructural, more study the mechanism that the acupuncture treats the acute cerebral nfarction. Methods (1)40 rabbits were randomly divided into 4 groups:The sham-operation group' neck was cut open, sew up after being separated the total artery in neck; the model group's net was cut open open , weared the line and ligated, not injected into the embolism, not pricked;the normal EA group was not cut open and pricked; the model EA group was pricked after being built the model.(2) Treatment methodThe model EA group was treated every 12 hours, 30 minutes each time.(3) Index examinationThe sham-operation group and the model group were examined after operation 1 12 24 48 hours, the CT was uesd to record each animal's brain area CT value blood-supplied by the middle cerebral artery, time-density curve was portrayed. The model EA group was examined after operation 1 12 24 48 hours, being pricked for 0.5 hours before examination.The normal EA group was examined once after being prinked for 0.5 hours, the normal group was examined at any time,whose recording method was the same to above. (4) The electron microscope observationA rabbit was taken each from the normal group the sham-operation group and the EA treatment group. The brain tissue of the middle cerebral artery blood-supplied area of each group was taken after 12 hours of operation.The changes of super microstructure of blood-brain barrier were observed and relative radiographs were taken. Experiment result1 At each phase ,the dynamic curve wave amplitude difference of the model group had obvious difference from the sham- operation group's, the model EA group had obvious difference from the model group's.2 At each phase,the time interval difference of the dynamic curve to the peaking of the model group had obvious difference from the sham- operation group's, the model EA group had obvious difference from the model group's.3 The BBB of the sham- operation group was not destroied in the electron microscope,while the BBB of the model group was destroied acutely.The BBB of the model EA group was destroied lighter than the model group's.ConclusionThe EA has obvious protection function to the acute cerebral infarction rabbits' blood-brain barrier.The possible mechanism is that EA can improve the brain flow effectively, improve the condition of the lack of the blood and oxygen in cerebra , reduce the degree of the hydrocephalus resulted from the attacks of cerebral , and accordingly reduce the breakage to the blood-brain barrier.