Experimental Study On Effects Of Electroacupuncture On Acute Cerebral Infarction In Ischemic Brain Tissue Monoamine Neurotransmitters

Objective:Cerebrovascular disease is a common and frequently occurring illness, disease with the feature of high disease incidence, high mortality rate and high mutilation rate. A great deal of clinical researches and empirical studies show that acupuncture has unique curative effect on this disease. To approach its' mechanism of action, we carried out this experiment. The SD rats were objects in this experiment. We made rat focal cerebral ischemia model with middle cerebral artery occlusion using a thread block technique. We gave a mark of the neurologic impairment in rat and detected the contents of NE,DA,5-HT in rats' ischemia brain tissue by electro-acupuncture(EA) on Baihui(DU 20) and Shuigou(DU 26) acupoint. In this way, we are trying to explore the mechanism of acupuncture therapy in acute cerebral infarction.Methods:40 SD rats were selected. Each rat's body weight was from 180g to 230g. They were randomly divided into four groups, each group with 10 rats: normal group, sham operation group, model group and model EA group. There are 10 in each group. Normal group rats were not provided treatment. Model group were made focal cerebral ischemia model with middle cerebral artery occlusion using a thread block technique. Model EA group was treated by EA on Baihui and Shuigou with continuous wave of 100 vices per minute frequency after the rats woke up. The treatment continued for five days, one vice for 30minutes per day. Then, we marked of the neurologic impairment in the rats and detected the contents of NE,DA,5-HT in rats' ischemia brain tissue.Results:1. focal cerebral ischemia rats with right middle cerebral artery occlusion using a thread block technique had some degree behavior disorder to some degree, with Powerless palpebra frontalis, levo- circling objective signs, and their left forefoot flection couldn't expand thoroughly. This showed that the CI models were made succeeded. After treated by EA, neurologic defect objective signs of rat improved obviously, Compared with model group, there was significant difference (P＜0.01).2. Compared with sham operated group and normal group, the contents of NE,DA in rats' ischemia brain tissue in model group increased greatly(P＜0.01). This result implyed that the contents of NE,DA in ischemia brain tissue had abnormality variation after CI. Compared with model group, the contents of NE,DA in rats' ischemia brain tissue in model EA group decreased greatly (P＜0.01).3.Compared with sham operated group and normal group, the contents of 5-HT in rats' ischemia brain tissue in model group increased greatly(P＜0.01). This result implyed that the contents of 5-HT in ischemia brain tissue had abnormality variation after CI. Compared with model group, the contents of 5-HT in rats' ischemia brain tissue in model EA group decreased greatly (P＜0.01)..Conclusion:1. The neurologic impairment of rats with crerbral ischemia improved obviously by EA on Baihui(DU 20) and Shuigou(DU 26) acupoint, indicating that EA can rapair cerebral ischemia damage.2. The contents of NE,DA in rats' ischemia brain tissue increased greatly in model group, it indicated that monoamine transmitter metabolic disorder in the brain after cerebral infarction.3. The contents of NE,DA in rats' ischemia brain tissue decreased greatly in EA group, The possible mechanism of action is that EA can adjust the monoamine transmitter metabolic disturbance, decrease the coup injury to ischemia neure, making a good contribution to reject the damage of cerebral ischemia.