Senate Surgery Polysaccharide The Gmps The Preliminary Study

Radix ginseng rubra and Rhizoma atractylodis macroceophaiae are topic traditional Chinese medicines. This research is on the base of traditional Chinese medicine-theory and pharmacodynmics study, using modern analysis technology find GMPS having effect on pathology of pregastric cancer from Radix ginseng rubra and Rhizoma atractylodis macroceophaiae and initially research on GMPS extracting, purifying method, physical-chemical character and component analysis of the function parts. The content of this research:Use water boilingRadix ginseng rubra and Rhizoma atractylodis macroceophaiae polysaccharide and get 4 parts precipitated by ethanol. Study the pharmacodynamics of these 4 parts respectively to screen effective parts GMPS which is effective to pregastric cancer.Study on GMPS extracting condition, after the medicinal materials extracted by ethanol. Use the quality of GMPS as the target to investigate water-extracting condition of GMPS with taking orthogonal L9 (3)4 design. Determinate the best water-extracting procedure: add 6 time water to boil 3 times, 1.5 hours each and combine with ethanol precipitated method. The 30%-60% ethanol precipitated parts is GMPS. The same method collects GPS, MPS, the ethanol precipitated parts same as GMPS from Radix ginseng rubraRhizoma atractylodis macroceophaiae.Tentative study on GMPS character: grayish-white to gray powder, odourless. Investigate hydrolysis condition. Use TLC, PC to initially probe into to components GMPS contain. Compare it with GPS and MPS. The result shows that GMPS contains Gal, Xyl, Rha, Gal A; can' t be sure whether have Ara, Glu, Man; can' t know the source of the parts in GMPS.Choose method of TCA by comparing with deproteinization method. After deproteinization GMPS through DE-52 cellulose column chromatography we can get three secondary components. The same way separates GPS, MPS. Analysis the secondary parts in GMPS by infrared spectrophotometer and compared with secondary parts the same wash parts in GPS, MPS. Result shows that the component in GMPS is different to GPS, MPS which include that mixed-boiling polysaccharide differ to separate-boiling polysaccharide mixed together.