| Massive clinical and animal experimental material indicated that, QINGKAILING'seffective ingredients have accurate effection on ischemic cerebrovascular disease. Thepreliminary experimental study showed that, QINGKAILING's effective ingredients such as thecholic acid, Jasminoidin, baicalin, and nacre play a vital role on the treatment of stroke. Thisarticle researchs on the mechanism and the character of their compatibility, in order to reveal itsbiology material of "detoxification and dredging collaterals", initially discusses the rule of theChinese native medicine compound prescription compatibility and provides evidence on theexperiment for the research modernization Chinese native medicine preparation. This topic includes two parts: the document research and the experimental study, the formeris about the relationship between proinflammatory cytokine and ischemic cerebrovasculardisease and the research survey of the Chinese medicine treating ischemic cerebrovasculardisease. The later studies the mechanism and the compatibility character of QINGKAILING'seffective ingredients blocking the inflammation response by the rat MCAO model. The resultsare as following: 1. Pathology observation of QINGKAILING's effective ingredients blocking theinflammation response Through hematoxylin and eosin staining, it can be seen that the brain organizationalstructure is clear and integrity, the cerebral cortex nerve cells are crowded and its arrangement isneat. Neuron plasm is rich and palely dyes, nucleus is on the core and the nucleolus is clear.Blood vessel endothelial cells are integrity and connected closely. The adhesion and Impactionof cells have not been seen. The organization around blood vesse is compact. The phlogocyte'sinfiltration has not seen in the Cortex (picture 1) in the sham-operated group. After 12h and 24h of ischemia, obvious damages can be seen in the operated groups. Wecan see that neurons are deleted obviously in the damaged cerebral cortex and its density reduces,stroma is edema and rarefaction. The nerve cell plasm is concentrated and dyed red. The nucleusis shrunk. Glial cells are proliferating. The gap around the vessel increases. The phlogocytegathers to vessel. The endothelial cell suffers injury, the partner sees in the blood vessel thepolymorphonuclear white blood cell adherency and Impaction. In the brain organizationobviously disperses in the lymphocyte infiltrates (picture 2-3). The groups administered with QINGKAILING's effective ingredients have the varyingimprovement on pathological changes respectively. The nerve cell swelling, karyopyknosis, thespongiocyte proliferation swelling, the blood pipe wall twists, pathological change and so onaccumulation has the change for the better, the neutral granular cell and the lymphocyte infiltratethe reduction, You Yique blood 12h gathers the side, the cholic acid, the mother-of-pearl functionis more remarkable (picture 5-14). The neuron specificity enolase (NSE) can"leak off" from the neuron after the cerebral英文摘要 -5-ischemia. It causes the content of NSE in the blood and cerebrospinal fluid rising. It had beenproved that the content of NSE in the serum has positive correlation with the infarct area aftercerebral ischemia. At ischemia 12h, nacre, cholic acid and their compatibility can reduce thecontent of NSE in the serum. Whereas Jasminoidin and baicalin have on effect on it. At ischemia24h, all groups administered with QINGKAILING's effective ingredients have on effect onNSE. It indicated that nacre, cholic acid and their compatibility could reduce the content ofNSE.so we can conclude that nacre, cholic acid and their compatibility have neuroprotection, buttheir mechanism is still to be discussed. 2. Mechanism discussion of QINGKAILING's effective ingredients suppressingproinflammatory cytokine harmful function In the study of pr... |
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