Basic Research Of Biological Treatment Of Endometriosis

To study the mechanism of cytokine IL-1 and its type II receptor on endometriosis, reverse transcription polymerase chain reaction(RT-PCR) and western blot were resorted to demonstrate the expression of IL-1 RII in normal endometrium, eutopic and ectopic tissues of endometriosis. The results showed that compared with B-actin, the expression of IL-1RII mRNA in transcription level in normal endometrium (0.8736 + 0.0972) was markedly higher than that in eutopic (0.4674 + 0.2324) and ectopic (0.4750 + 0.2688) tissues of endometriosis, the expression of IL-1RII protein in translation level in normal endometrium was markedly higher than that in eutopic and ectopic (2.057118 + 0.838522>l>0.630981 + 0.591847) tissues of endometriosis. It indicated that IL-1R II may play a role in local immunological regulation of cytokines in endometrium and the lower expression of IL-1RII gene and protein implied that may be associated with the occurrenc of endometriosis.To further study the regulative mechanism of IL-1 RII on the tissues and cells of endometriosis and to provide evidence for the clinical biologic therapy of endometriosis, human IL-1RII cDNA was amplified by RT-PCR from peripheral blood mononuclear cells (PBMCs) and confirmed by sequencing. Through prokaryotic expression, we found that E.Coli BL21 mostly expressed the protein (Mr 45,000) under IPTGinduction. Expressed product was detected by Western blot, so it was immune active. Human IL-1RII cDNA was subcloned into retrovirus vector LZRSPBMN. And we confirmed that IL-1RII could be expressed in eukaryocyte by immunohistochemical staining. This provided a necessary condition for the research of the mechanism how IL-1RII regulates IL-1.At meantime, we successfully established ICR mouse endometriosis model with their own endometrial implantation. This animal model made a contribution to the further research .In conclusion, the lower expression of IL-1RII implied it may be associated with the development of endometriosis. Cloning, analyzing the expression of IL-1RII and setting up ICR mouse endometriosis model may provide essential materials for the further studies on cytokines gene transfection of endometriosis cells, especially the study on the mechanism of cytokines network associated with whole cellular and humoral immunity on endometriosis. With these, we might find new target and pathway for biological therapy to endometriosis.