Psoriasis Cd4 ~ + Of Cd8 ~ + T-lymphocytes To Keratinocyte Cell Ki67, C-myc And Bcl-xl Protein Expression In Experimental Research

Objective: To reveal the action of psoriatic T lymphocytes and CD4+T,CD8+Tlymphocytes in keratinocytes Ki67,c-Myc and Bcl-xL protein expression and thesignificance in the pathogenesis of psoriasis.Methods: Density gradient centrifugalization and absorption technique wererespectively applied to detach peripheral blood mononuclear cells(PBMC) andperipheral blood lymphocytes(PBLC),T lymphocytes were got by Nylon postabsorption method, and CD4+T and CD8+T lymphocytes divided with anti- CD4 andanti-CD8 antibody by alexin cytotoxic method. Keratinocytes were cocultivatedrespectively with psoriatic T lymphocytes,CD4+T lymphocytes and CD8+Tlymphocytes for 72h in comparison with those cocultivated with normalperipheral T lymphocytes,CD4+T lymphocytes and CD8+T lymphocytes.Immunohistochemistry technique were performed to detect the expression ofKi67,c-Myc and Bcl-xL proteins.Resultes: There were significant overexpression of Ki67,c-Myc and Bcl-xLproteins in keratinocytes cocultivated with psoriatic T lymphocytes,CD4+Tlymphocytes and CD8+T lymphocytes. Expression of Ki67,c-Myc and Bcl-xLproteins in keratinocytes cocultivated with normal T lymphocytes ,CD4+Tlymphocytes and CD8+T lymphocytes were not significantly different comparedwith the uncocultivated group. The proteins expression in keratinocytescocultivated with psoriatic CD4+ T lymphocytes were higher than those with CD8+Tlymphocytes, while the difference between keratinocytes cocultivated withnormal CD4+ T lymphocytes and CD8+T lymphocytes is not statistically significant.Conclusion: Psoriatic T lymphocytes,CD4+T and CD8+T lymphocytes can inducekeratinocytes to proliferate abnormally. The overexpression of Ki67,c-Myc andBcl-xL proteins might be one of the important mechanisms. Psoriatic CD4 T +lymphocytes have much more significant influence on Ki67,c-Myc and Bcl-xLprotein expression of keratinocytes, which might be responsible for earlypsoriatic lesions formation.