Ketones Ingredients. Mileensis (mouth Of The Mountain)

Hepatitis B infection is a global public health problem, which often 1 eads to the development of chronic liver disease and hepatocellular carcin oma. Because of the participation of self-immunity of HBV, up to now, d espite of many existing antiviral and immunomodulatory agents in market,there are not still an drug effective against it. Now more and more peop le try to search effective ingredients against acute and chronic hepatitis. T. N.Ho et W.L shih contain effective anti-HBV components-xanthone and ol eanoic acid, which plays an important role in protecting liver and reducin g aminotransferase level. Pharmacological research indicated that flavonoid s in Swertia mileensis can strongly inhibit the liver damage caused by C Cl4, and have much strong inhibition against GAIN .Clinical trials have p roven extracts from Swertia mileensis have evidently curative effects on a cute jaundice hepatitis and chronic virus hepatitis, and they are of low to xicity, few side-effects and high safety II]. Our group have done systemati c research on Swertia mileensis T.N. Ho et W.L. shin, twelve compoundsbelonging to xanthone and an oleanoic acid were separated, extracted, pu rilled, and appraised systematically, at the same time their structures werecharacterized by spectral analysis.The dried plant of Swertia mileensis T.N. Ho et W.L. shih was grou nd and extracted (24h*3) with 95% ethanol, The solution were concentrat ed in vacuum to yield an extract. The extract was subjected to silica gel and alumina column chromatography, and twelve compounds were separate d. Their structures were elucidated by UV, IR, MS, 'HNMR and other s pectrum evidence.Results: There were 12 xanthone analogues named l-hydroxy-2, 3,4,5 -tetramethoxyxanthone( A-1); 1 -hydroxy-2,3,7-trimethoxyxanthone,( A-2); 1 -hydr oxy-2,3,5,7-tetramethoxyxanthone,(A-3);l,5-dihydroxy-2,3-dimethoxyxanthone,(A-4); 1,5-dihydroxy-2,3,7-trimethoxyxanthone,(A-5); l-hydroxy-2,3,5-trim eth3oxyxanthone,(A-6); 1, 5-dihydroxy-2,3,4,7-te-tramethoxyxanthone,(A-7); 1,8-di hydroxy-2,3,6-trimethoxyxanthone,(A-8); 1 -hydroxy-2,3,4,7-tetramthoxyxantho ne,(A-9);l,2,3>5-tetromethoxyxanthone,(A-10);l-hydroxyl-2,3,4,6-tetram-ethox yxanthone,(A-11); 1 -hydroxyl-2,3,6,8-tetramethoxyxanthone,(A-12);along witha common triterpenoid oleanolic acid (A-13).Conclusion Compounds A-l;A-3; A-4; A-5; A-7; A-12 were first isolated from S. mileensis.