Purple Swertia Chemical Composition And Hplc Fingerprint Of The Study

Swertia punicea Hemsl, a dried herb of Gentianaceae, is used as a folk traditional medicine in China for a long time .It is a folk traditional medicine against hepatitis, cholecystitis , pneumonia , pandemic influenza, Inflammation of bone et. The contents of this study belongs to the study of the material foundation of the compound prescribe ZI JIN GAN TAI anti- B liver virus that is funded by National Natural Science Fundation of China. Based on its pharmacology and clinical research, we have carrid on relevant experiments on it.The whole plants of Swertia punicea Hemsl was percolated by the ether , then petroleum ether fraction ,aether fraction, ethyl acetate fraction and water fraction were gotten one by one by means of solvent extraction. Afer the selection of activity of anti-hepatitis B virus 10 constituents were isolated from its active fractions- ethyl acetate fraction and water fraction-by various kinds of chromatographies.Seven of them were elucidated on the basis of UV, IR, NMR(¹H-NMR. ¹³C-NMR. HMQC, HMBC) and MS spectral data as follows: 1, 7, 8-trihydroxy-3-methoxyxan-thone (Ⅰ), 1,5,8-trihydroxy-3-methoxyxanthone(Ⅲ), 2-C- p -D-glucopyr-anosyl-1, 3, 6, 7-tetrahydroxyxanthone (Ⅳ) , Swertamarin(Ⅵ), 8-0- P -D-glucopyranosyl-1,5-dihydroxy-3-methoxyxanthone(Ⅶ), 5-0- β-D-glucop-yranosyl-1,8- dihydroxy-3-methoxyxanthone (Ⅷ), oleanolic acid(X).The study of the chromatographic fingerprint of Swertia punicea Hemsl has been set up by HPLC for 10 batches herbs collected in different districts. Chromatographic column: Kromasil C₁₈(250mm×46mm, 5μ)Mobile phase: methanol -0.05%aquaintance-acetonitrile. Gradientelution: methan 0%:0min 15%, 30min 55%, 45min 55%, 60min 65%, 90min 70%; acetonitrile%: 0-90min5%. Flow rate: 0. 8mlmin^-1Wavelength: 254nm. Column temperature: 30 ℃. Inject volume: 10μ1- 13 common peaks in The fringerprint of Swertia punicea Hemsl were indicated. The method is stable, well repeated and provides scientific indexes for the internal quality of Swertia punicea Hemsl under the state of nature.