| The morbidity and mortality of lung cancer are gradually occupying the first place of various kinds disease. Owing to the defect of effective therapeutic tool,especially the effective therapeutic tool of controling metastasis,it induces invariably the therapeutics failure.How to prevent and block up the metastasis is the key point in the therapy of lung cancer,and it's also the hot and difficult spot of lung cancer's investigation in all over the worldRecently it has been more and more reconstred that the relationship of metastasis with blood stasis and promoting blood circulation to remove blood stasis. Synthetizing the investigating progress and combining my advisor's study results in this field we may deem that the syndrome of blood stasis of TCM is a main cause of tumor metastasis. On account of the viewpoint, this study explores the syndrome of blood stasis status in advanced lung cancer patiens which the mortality is fairly high( the cause of death is mainly metastasis) , and researches the possible target which the drug for invigorating blood circulation and eliminating stasis interferes with the process.In the way ,we would develop the cognition in metastasis of lung cancer.1 Reviews1.1 Reviews oneAccording to the study of the antique literature, investigating advancement of tumor metastasis, syndrome of blood stasis and the principle of promoting blood circulation to remove blood stasis,we accordingly identified that syndrome of blood stasis is the entity of tumor metastasis, and the interfering effects of drug for invigorating blood circulation and eliminating stasis. In addition,we also draw off the existing problem and solving strategy.1.2 Reviews twoWe summarized the clinical and experimental investigation on differentiation of symptoms and signs for classification of syndrome of lung cancer. Concretely,we summarized the relationship of differentiation of symptoms and signs for classification of syndrome of lung cancer with the international TNM staging of lung cancer, patho-typing, lately and prostecdtive efficacy, protein and polypeptide, immunology ,blood rheology and elements. This review provided some document for the investigation on differentiation of symptoms and signs for classification of syndrome of lung cancer, and proposes the existing problem and solve strategy.2 Clinical research2.1 The investigation of distributing characteristics of blood stasis syndrome on 300 cases of advanced Stage Lung Cancer patiensThrough filling in the table ((syndrome of distributing characteristics of primary pulmanary carcinoma)) for stage III and IV patients,we observed the specific weight and distributing characteristics of syndrome of blood stasis in advanced lung cancer patiens. we also analyzed the distributing characteristics of syndrome of blood stasis in different patho-typing,different cell differentiation degree,different staging,dhTerent metastatic location, different complication of lung cancer and different KPS score. As the consequence showed, the constituent ratio of blood stasis syndrome increased significantly following the worse patho-typing degree, the cell differentiation degree,the occurrence of hepatic metastasis,opposite pulmonary metastasis, osseous metastasis and troisier sign. Through observing the frequency of occurrence, distributing characteristics of cardinal symptom and objective sign of syndrome of blood stasis, we discovered that the syndrome of blood stasis of lung cancer has the distinct feature. Accordingly,we ought to erect the diagnostic standard which can be qualified of syndrome of blood stasis. Only in that way can we guide the clinical diagnosis, the evaluation of therapeutic effect and the clinical trial of new drug of TCM preferably.2.2 The clinical observation of the different express of surface adhesive proteins on blood platelet between advanced lung cancer patiens and health adultsThe investigation showed the different expression of surface adhesive proteins CD36, TSP, CD63 and CD62 on blood platelet in stage III and IV lung cancer patiens and health adults by flow cytometry. We observed the different condition of their platelet activation status. As the consequence showed, the thrombocytic numbers of CD36*TSP\CD36 + TSP~,CD63+CD62\CD63"CD62* and CD63+CD62' of patients are higher than health adults. The thrombocytic mean fluorescence intensity of CD36 on CD36+TSP+ platelets and CD63 on CD62+CD63'platelets of patients are also higher than health adults. They showed significant difference. The thrombocytic numbers of CD36*TSP\CD63~CD62* and CD63+CD62* of stage IV patients are higher than stage III patients.The thrombocytic mean fluorescence intensity of CD62 and CD63 on CD62*CD63 'platelets of stage IV patients are also higher than stage III patients. And it all has significant difference. Thereby, we can deem that the platelets of advanced lung cancer patiens are in activated status, and it has significant difference compered with health adults. Furthermore, the thrombocytic activation condition of advanced lung cancer patiens are concerned with tumor staging and metastasis status.So we can presume that syndrome of blood stasis are not only one of carcinogenesismechanisms of lung cancer, but also one of essence of lung cancer spreading.3 Empirical study3.1 The detection of the influence of Elemene,PDGF-AB and TGF- p i on cellmultiplication of PG and PAa by MTT assayWe detected the influence of different dosage Elemene ,PDGF-AB and TGF- P, on cell multiplication of PG and PAa by MTT Assay. As the consequence showed, Elemene has inhibiting effect on PG and PAa cell line, and its IC50 to PG and PAa is about 75ug/ml and 37.5ug/ml. On the whole, the cytokine of PDGF-AB and TGF- p i which derived from platelets have not any inhibiting effects on PG and PAa32 The influence of Elemene in the invasion and metabasis related surface adhesive proteins on lung cancer cellWe used confocal microscopy observed the expression of the thrombocytic immune related antigen CD42a, TSP and CD9 on high metastasis lung cancer cell line PG and low metastasis lung cancer cell line PAa We also used flow cytometer observed the expression of the thrombocytic immune related antigen CD42a, TSP and CD9 on PG and PAa, and the alter of the expression of CD42a,TSP and CD9 when the two cell lines were incubated with different level ElemencAs the consequence showed, we saw that the CD42aTSP and CD9 expression adqulis on cell surface by confocal microscopy. By flow cytometer, we saw that the positive cell numbers and mean fluorescence intensity of CD9 on PG cell are lower than on PAa cell, but the positive cell numbers and mean fluorescence intensity of TSP and CD42a are all higher than on PAa cell. Thus,the results confirmed the metastasis capability of PG is higher than PAa From general tendency, Elemene increase the expression of CD9 and decreased the expression of CD42a and TSP on PG and PAa cells. It is one of the possible molecule mechanisms of Elemene inhibiting lung cancer metastasis.33 The experiment of the recombination basal lamina invasived by lung cancer cell line PG and PAa ,and the influence to that by Elemene ,PDGF-AB and TGF- p iWe observed the invasion to recombination basal lamina of lung cancer cell line PG and PAa, the influence of different level Elemene to that process, we also observed the influence on invasion of PDGF-AB and TGF- P i incubated with PG and PAa cell and the interference of different level Elemene to that process. As the experimental result showed, the invasive capacity of PG cell is higher than PAaThe invasion charcter is different from two cell lines. We also discovered that Elemene has inhibiting... |
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