Specific Cyclooxygenase-2 Inhibitors Celecoxib Vitro Induces Gallbladder Carcinoma Cell Line Gbc-sd Apoptosis And Fk506 On The Treatment Of Severe Acute Pancreatitis Rats

AIM:The current studies were designed to study the effects of inhibiting proliferation and inducing apoptosis of celecoxib on human gall bladder cancer cells in vitro. After oberserving the inhibitive and inducing effects of celecoxib at various concentrations, we discussed the mechanism behind those phenomena of specific inhibitor of COX-2 in favor of clinical practice primarily.METHODS: 1 Through counting method to set out growth curve of gall bladder cancer cells, and understand its basic characteristic.2 The gall bladder cancer cell were cultured with celecoxib at various concentrations (0, 40, 80,160&320μmol/L). Growth suppression was detected by MTT colorimetric assay, cell apoptotic alterations were evaluated by flow cytometry (FCM). 3 The gall bladder cancer cells were cultured with celecoxib(160μmoL/L), and had been stained by Hoechst33258 and TUNEL method.RESULTS:1 Through counting method to set out growth curve of gall bladder cancer cells.2 The inhibition of proliferation on gall bladder cancer cells can be observed. And the inhibitive effect was dose-dependent and time-dependent. Apoptotic cells were observed by FCM.3 By stain of Hoechst33258 and TUNEL, we observed apoptosis. CONCLUSION:Celecoxib...