Lentinan Bone Marrow Suppression, Anemia Mouse Hematopoietic Regulation

Objective To examine the effect of LNT (lentinan) on aplastic anemia mice Periphery blood, BMC amount and cell cycle; also evaluate the influence of hemopoietic progenitor cell unit and K562 cultivated in vitro affected by LNT and variety of cytokin IL-6 excreted by bone marrow stromal cell, for Investigating the hematopoietic regulation effect of LNT and study the modern mechanism of Ying-Wei tongyuan, Ying-Wei jiaohui addressed by Chinese traditional medical theory.Methods 1 BalB/c Mice were established AA model and divided into four groups, treated by different concentration LNT, To count the amount of WBC, RBC, Hb, PLT and bone marrow nucleated cells , Observe the cell cycle by FCM (flow cytometry)2. Cultivated BMC extracted from aplastic anemia mice in vitro and account the unit of hemopoietic progenitor cell, and evaluate the different effect changed by different concentration of LNT in vitro.3 Adope enzyme linked immunosorbent assay (ELISA) to investigate the different concentration of cytokine IL-6 excrete by bone marrow stromal cell cultured in vitro affected by different concentration LNT. Results Experimental results indicate: Within the range of certain concentration:1 LNT can increase the amount of WBC, RBC and BMC but not include blood platelet, also stimulate more BMC into cell cycle, promote. BMC...