Spectroscopy Of Human Serum Albumin Determination And Rhodamine B, And Fluorescent Yellow Fluorescence Spectra

Human serum albumin (HSA) is the major protein component of adult plasma. It is of function as a carrier for diverse substances and is largely responsible for maintaining normal osmolarity in the plasma, and used in medicine such as shok, edema and hypoproteinemia. So, the detection of HSA is the important phase in biochemistry and clinical medicine. Meanwhile, Spectrophotometry owns the virtue of simplicity, accuracy, sensitivity and relatively low cost. Due to the above researches, Spectrophotometnc method, second older scattering method , anti-second order scattering method and fluorescence method were studied to determine the HSA in the paper. The fluorescence spectra of RhB and HFIn were studied, too. The outlines are as follows:1 In a Clark-Lubs buffer at pH 3.5, the absorptivity of calcein is reduced by the interaction of HSA and calcein. Base on the above, Spectrophotometnc Determination of HSA was developed. The linear range is 1.14-17.1mg/L, with the detection limit to be 0.94mg/L. The average recovery is 101 percent and the relative standard deviation is 4.75 percent.2 In a Clark-Lubs buffer at pH 3.5, human serum albumin (HSA) can bind with Calcein to form new products, resulting in the great enhancement of second older scattering(SOS) and anti-second order scattering(ASOS) spectra. The enhanced intensity of SOS and ASOS is proportional to the concentration of HSA in the range of 0.11-1.37 mg/L and the detection limits are 0.061mg/L and 0.057mg/L. The average recoveries are 96.7 percent and 108 percent and the relative standard deviations are 2.0 percent and 1.9 percent.3 In a Clark-Lubs buffer at pH 3.5, human serum albumin (HSA) can bind with fluorescein to form new products, resulting in the great enhancement of second older scattering(SOS) and anti-second order scattering(ASOS) spectra. The enhanced intensity of SOS and ASOS is proportional to the concentration of HSA in the range of0.057-0.68 mg/L and the detection limits are 0.033mg/L and 0.054mg/L. The average recoveries are 98.8percent and 103 percent and the relative standard deviations are3.16 percent and 4.29 percent.4 In a Clark-Lubs buffer at pH 7.8, calcein can form its dimer under the effect of cation surfactant and its fluorescence intensity decreases, however, addition of HSA causes the dimer to depolymirize and the system fluorescence increases. Base on the above principle, we established the fluorescence method to determine HSA. The linear range is 0.29-39.8mg/L and the correlation coefficient is 0.997, with the detection limit to be 0.13mg/L. and the average recovery to be 96.7 percent.5 In the paper, the fluorescece spectroscopy of RhB was studied through the density of RhB, the polarity of solvents, the magnitude of pH and the enhancement of surfactants. Besides, the mechanics of the above four aspects were discussed.6 In the paper, the fluorescence spectroscopy of HFIn was studied through the density of HFIn, the polarity of solvent, the magnitude of pH and the enhancement of surfactants. Besides, the mechanics of the four aspects of above were discussed.