Discussion Of Traditional Chinese Medicine For The Effect And Mechanism Of The Compound On Experimental Diabetic Rats, Neurons In The Visual Pathway Of Pathological Damage

Objective: To observe the histomorphological pathology change of the optic pathway neuron in the experimental diabetic rat, and to probe into the mechanism of Bu Shen Huo Xue Zhongyao Fufang (补肾活血中药复方) to prevent from the lesion of the optic pathway of the experimental diabetic rats.Methods: The diabetic SD rats were made by abdominal streptozotocin-injecting. Experimental rats were divided into normal group, negative control group, positive control groupⅠ , positive control groupⅡ, Bu Shen Huo Xue Zhongyao Fufang groups (lower dosage, moderate dosage, high dosage) at random. And then The Nissl body, NT-3, SynI, Bcl-2 in retina, EGB and VC 17 area were checked by Niss staining method and immunohistochemical staining method. The experimental data were done by SPSS 11.0 software package. Results: (1) The positive expressions of Nissl body, NT-3, Syn I and Bcl-2 in retina, EGB and VC of the rats in the negative control group after 6 months were decreased in various degrees ( P<0.01 , 0.05). (2) in comparison with the expressions of Nissl body, NT-3, Syn I and Bcl-2 in retina, EGB and VC of the rats in the negative control group, those of the rats in the Bu Shen Huo Xue Zhongyao Fufang groups (lower dosage, moderate dosage, high dosage) were enhanced, especially with the high dosages(P<0.01).Conclusions: The experimental diabetic rats which were in high blood glucose condition for 6 months, the optic pathway neuron had the varying degree of the recessive change. By means of strengthening the expression of NT-3, Syn I, bcl-2 in optic pathway neuron to inhibit its recessive change of diabetic rats, Bu Shen Huo Xue Zhongyao Fufang could improve the releasing and regulating function of neurotransmitter to reduce the lesion of diabetic optic pathway.