The Effect Of BuJingYiShi Tablets On The Expression Of P-FoxO1 And IKK2 In The PI3K/Akt Pathway And Nissl Body In PVC In Rats With Chronic EIOP Model

Objective:To observe the effect of traditional BuJingYiShi tablets expression of niss body and PI3K/Akt signal pathway's p-FoxO1 and IKK2 on primary visual cortex(PVC)damage in SD rat model of chronic elevated intraocular pressure(EIOP),and explore the mechanism of it initially.Methods:By unilaterally cauterizing 3 episcleral vessels establish the rat model of chronic EIOP 30 SD rats were divided into 3 groups randomly:control group,model group and treatment group.After given BuJingYiShi tablets or normal saline for 8 weeks,the rats were put to death.To observe the effection of BuJingYiShi tablets on the EIOP SD rats model's intraocular pressure(IOP),niss body and PI3K/Akt signal pathway's p-FoxO1 and IKK2 in primary visual cortex.Results:The establishment of chronic EIOP model in SD rats by unilaterally cauterizing 3 episcleral vessels can increase the IOP of SD rats,and it can last at least8 weeks(at the end of the experiment),compared with that before modeling,the difference was statistically significant(P<0.05),but he difference was not significant IOP in model group(P>0.05).The IOP of the model after 8 weeks is compared to that after the model,there was no significant difference IOP in model group(P>0.05),and IOPintreatmentgroupdecreasedslightly(P<0.05).Thetotalarea(72206.00±9487.988 S/?m~2),integrated optical density(7024.105±327.763)and mean optical density(137.009±5.612)of nissl bodies in model group lower than control group(126371.50±9549.111 S/?m~2,10635.126±785.643,174.362±10.614)and treatment group(107786.17±11642.358 S/?m~2,8538.564±427.210,152.499±4.754)(all P<0.05);The total area,integrated optical density and mean optical density of nissl bodies in treatment group lower than control group(P<0.05).The total area(0.0920±0.0142 S/?m~2),integrated optical density(103665.448±11059.419)and mean optical density(211.706±2.308)of p-FoxO1 in model group higher than control group(0.0486±0.0119 S/?m~2,29403.664±9808.723,200.279±1.720)and treatment group(0.0635±0.0087 S/?m~2,66452.646±12460.639,204.566±1.717)(all P<0.05);The total area,integrated optical density and mean optical density of p-FoxO1 in treatment group lower than control group(P<0.05).The total area(0.1091±0.0236S/?m~2),integratedopticaldensity(19593.710±3052.406)andmeanoptical density(211.965±5.743)of IKK2 in model group higher than control group(0.0321±0.0085 S/?m~2,4633.886±1353.392,186.747±10.488)and treatment group(0.0662±0.0131 S/?m~2,9397.547±2284.144,200.151±9.630)(all P<0.05);The total area,integrated optical density and mean optical density of IKK2 in treatment group lower than control group(P<0.05).Conclusion The operation of chronic EIOP model in SD rats is simple,the effect of increasing IOP is stable,and the high IOP is not less than 8 weeks.BuJingYiShi tablets can protect the visual function from elevating IOP,by increasing content of nissl bodies,reducing the expression of p-FoxO1 and IKK2 in PVC.