Long-term High Fat Feeding In Normal Rats Glucose-stimulated Insulin Secretion And Liver 11¦Â-hsd <sub> 1 </ Sub> Mrna Expression Of The Impact

[Objective] To observe the effect of long-term high fat feeding with normal total calorie intake on glucose stimulated insulin secretion and plasma adiponectin in normal rats in order to probe the association of dietary fat with type 2 diabetes mellitus(T2DM).[Methods] 35 healthy male Wistar rats(8 weeks old) were selected and divided into two groups including routine feed (NC,n=16) and high fat diet(HF,n=19).The total calorie ingestion of each rat per day was similar and feeding duration was 24 weeks. Body weight was monitored during feeding. The insulin secretary function of β cell, especially acute insulin response(AIR),was assessed by intravenous glucose tolerance test(IVGTT) in 32-week-old rats. The levels of plasma adiponectin were tested by radioimmune assay and HOMA-IR was calculated by the method: HOMA-IR=FBG×FINS/22.5.[Results]1. Body weight and visceral fat mass: Compared with NC , there were significant differences in body weight and visceral fat mass of HF. The rats taking HF had significantly higher body weight[(559.3±58.8)g vs (503.1± 36.2)g,p<0.005]and developed severe visceral obesity [visceral fat rate(9.3 ± 2.5)% vs (4.8 ±...