Orchid Bean Hepatoprotective Effects Of Particles On Experimental Liver Fibrosis In Rat Timp-1 Expression

Objective hepatic fibrosis is the common pathological stage in the process of the chronic liver disease caused by variant etiology. It is associated with the production and deposition of extracellular matrix which is regulated by MMPs and TIMPs, especially by TIMP-1. TIMP-1 inhibiting MMPs is elevated while the liver is damaged, and leads to the decreasement of the ECM degradation and the increasement of the deposition which is directly associated with hepatic fibrosis . The incidence of the chronic hepatitis and hepatic fibrosis is 59.36%, among which there is about 25%-40% would develop the liver cirrhosis. The major factor in China was the epidemic HBV. Liver cirrhosis is always thought to be the irreversible pathological state, while hepatic fibrosis reversible one. How to stop the progression of the hepatic fibrosis seems critical to the therapy. Up to date, the western medicine has not efficient drugs to deal with chronic liver disease while Chinese medicine and herbs has its advantage in treating it.Methods the research guided by the theory of the traditional Chinese medicine investigate the cause, the mechanism, the pathology and the therapy. We hold the opinion that the hepatic fibrosis is caused by the dampness heat and epidemic toxin, the irregulation of the diet, the imbalance of the emotion. The basic mechanism is the impairment of the liver, kidney, spleen, Qi is silted with Xue. The pathological factor include the dampness, heat, toxin, siltation etc. The principle of the therapy could be divided into two parts, the early stage of the disease is based on soothing the liver and strengthening the spleen, clearing away the heat-evil and eliminating wetness ,regulating the flow of qi and activating blood circulation ,while the advanced stage nourishing the liver and invigorating kidney, supplementing qi and strengthening the spleen, activating blood circulation and eliminating wetness. The Landou Hepatoprotecting Granule from the celebrity professor zouliangcai has the conclusive effect on the disease after many years clinical research. With the modern advanced technology, this research aimed to elevate effects of Landou Hepatoprotecting Granule composed with completely Chinese herbs on the experimental hepatic fibrosis in rats, compared with results from Colchicine. By detecting serum biochemical changes, routine pathologic changes and immune pathological changes and others, the exact effects of Landou Hepatoprotecting Granule on protecting liver from hepatic fibrosis and its therapeutic mechanism may be elucidated.Result Landou Hepatoprotecting Granule has a good effect on preventing rats from the damage caused by carbon tetrachloride. It can decrease the production of TIMP-1, reduce the deposition of the extracellular matrix, and prevent liver from hepatic fibrosis. Studying in the Chinese herbs aimming to prevent from hepatic fibrosis is established while inchoate clinical diagnosis on hepatic fibrosis would also be benefited.