Experimental Study Of Three-dimensional Reconstruction Of The Foreskin Abnormal Blood Vessels. Rabbit Hypospadias

Objective To establish a rabbit model of hypospadias finasteride to further stadying molecular mechanisma of hypospadias etiology. And realized the 3DR of blood vessel variation of hypospadias animal model.Methods: forty pregnant new zealand rabbits were randomly divided into four groups. Finasteride was fed with 0days(group A),4 days (group B),5 days (group C), 6 days (group D),7 days (group E) which begin at GD(gestation days) 19, 10mg.kg^-1.d^-1.The appearance of penis and the position of urethras orifice were exmamined to observe hypospadias on postnatal day 35; the second observation was performed to observe retained testicle and the Severity of hypospadias on postnatal day 70, all of the male rabbits were anatomized to observe the development of didymus. 10 weeks after birth, cut the penis of rabbit, put them to be continuous sectted and HE staining. Realized the 3DR image of blood vessel with photoshop7.0 and 3DMAX8.0 software.Results: Hypospadias was seen in group A(0),group B(22.2%),group C(95.5%),group D(85.2%),group E(100%),and Severity of hypospadias can be divided into 3 degrees. Retained testicle was seen in group A(0),group B(0),group C(36.4%),group D(40.7%),group E(73.3%). The 3DR image of blood vessel in type A hypospadiasis is one-bole type, type B hypospadiasis is two-boles type, type C hypospadiasis is NET-like type.Conclusion: The experimental model of hypospadias induced by finasteride is stable. The penis blood vessel of hypospadias animal model have variation of blood vessel. The 3DR image of blood vessel in three types hypospadias is different .