Intervention Effect. Mongolian Medicine Chapter Ancient -3 Soup Rats With Chronic Renal Failure Induced By Adenine

Objective To study the therapeutic effects of Traditional Mongolian Medicine Zhanggu- Ⅲ on adenine - induced chronic renal failure (CRF) in rat and explore the mechanism of Zhanggu- Ⅲ in preventing and treating CRF. Methods Sixty wister rats were randomly divived into six groups : normal group , model group , Zhanggu- Ⅲ high dosage group(ZG- Ⅲ H), Zhanggu — Ⅲ middle dosage group( ZG - ⅢM), Zhanggu — Ⅲ low dosage group (ZG- Ⅲ L) ,and Niaoduqing group, each group had 10 rats. Each rat in model group,ZG-Ⅲ groups and niaoduqing group was infused with Adenine(200·Kg-1·d-1) into stomach . in the same time , each rat in ZG - Ⅲ group was also infused with high(20g· kg-1 · d-1), middle(10g·kg-1·d-1) ,low(5g·kg-1·d-1) dosage forms ZG- Ⅲ into stomach for 24 days ; the Niaoduqing group was given Niaoduqing liquid. The content of blood urea nitrogen (BUN),serum creatinine (Scr) ,biochemical indexes (Ca2+ ,K+ ,Na+ ,P5+ ) .indexes of oxygen free radicals(SOD, MDA), and changes in histopathology were observed after 24 days. Transforming growth factor -β₁ (TGF -β₁ ) expression were examined by immuohistoche-mistry. Results in ZG - Ⅲ treated group , the renal function is obviously lower than that in model group(P<0.05). ZG- Ⅲ increased the level of Ca2+ ,reduced the levels of P5+ and K+ (P<0.01), TGF-β1 expression level is lower compared with model group, ZG- Ⅲ had the effect of increasing the level of SOD and reducing the level of MDA(P<0.05). Conclusion Traditional Mongolian Midicine ZG - Ⅲ can protect renal function of adenine - induced CRF rats, the mechanism may be relative to suppressing the expression of TGF-β₁ and antioxidation.