| Objective: Magnolol (Mag) is a Chinese herb that has potent anti-inflammatory effects. Our aims were to investigate the effects of magnolol in the treatment of acute lung injure and to assess its influence on the expression of tumor necrosis factor alpha (TNF-α) , interleukin (IL)-6 in blood serum and nuclear factor-κB (NF-κB) in lung tissue in acute necrotizing pancreatitis (ANP) in rats.Methods: ANP was induced by retrograde infusion of 5% sodium taurocholate into the biliary pancreatic duct of Sprague-Dawley rats. Fifty-six rats were randomly divided into three groups: sham operation (SO) group, ANP 6 h, 12 h, 24h groups, and Mag 6 h, 12 h, 24h groups. The gene expression of NF-κB in lung tissue were assayed using fluorescence quantitative polymerase chain reaction (FQ PCR). The activity of serum amylase (Amy) , TNF-α and IL-6 was determined. Pathological changes of the pancreas and lung were observed.Results: Compared with that in SO group, The gene expression of NF-κB,TNF-α, IL-6 and serum Amy in ANP group were increased greatly (p<0.01), . Mag treatment resulted in lower gene expression of NF-κB, TNF-α ,IL-6 and serum Amy levels (1.30±0.14 vs 1.84±0.56, P<0.05; 43.15±5.93 vs 76.18±8.21, P<.01; 40.73±8.21 vs 65.78±5.06, P<0.01; 6273.33±1492.87 vs 11315.00±1424.53, P<0.01, respectively) compared with that of the ANP group. Mag treatment showed great histological improvement in the lung tissue but no relief in the pancreatic tissue.Conclusion: Mag may decrease the expression of the proinflammatory factors as NF-Kb, TNF-α and IL-6 and relieve the acute... |
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